Selection of Aptamers Specific for Adipose Tissue

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Jun 5

PMID 22662223

Citations 19

Authors

Jun Liu

Huixia Liu

Kwame Sefah

Bo Liu

Ying Pu

Dimitri Van Simaeys

Weihong Tan

Affiliations

Soon will be listed here.

Abstract

Background: Obesity has reached epidemic proportions, affecting more than one tenth of the world's population. As such, adipose tissue is being increasingly recognized as an important therapeutic target for obesity and related metabolic disorders. While many potential targets of adipose tissue have been established and drugs developed, very few of those drugs specifically target adipose tissue without affecting other tissue. This results from a limited knowledge of both cell-surface markers and physicochemical traits specific to adipocytes that might otherwise be exploited by circulating drugs.

Methodology/principal Findings: Here we report the use of cell-SELEX technology to select two aptamers that can specifically recognize mature adipocytes: adipo-1 and adipo-8. Adipo-8 shows high affinity for differentiated, mature 3T3-L1 adipocytes with a K(d) value of 17.8±5.1 nM. The binding was sustained upon incubation at 37°C and insulin stimulation, but was lost upon trypsin treatment. The binding ability was also verified on frozen tissue slides with low background fluorescence and isolated adipocytes.

Conclusions/significance: Aptamer adipo-8 selected from a random library appears to bind to mature differentiated adipocytes specifically. This aptamer holds great promise as a molecular recognition tool for adipocyte biomarker discovery or for targeted delivery of molecules to adipocytes.

Citing Articles

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Chemically modified aptamers for improving binding affinity to the target proteins enhanced non-covalent bonding.

Chen Z, Luo H, Gubu A, Yu S, Zhang H, Dai H Front Cell Dev Biol. 2023; 11:1091809.

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Adipose-targeted triiodothyronine therapy counteracts obesity-related metabolic complications and atherosclerosis with negligible side effects.

Chen K, Cheong L, Gao Y, Zhang Y, Feng T, Wang Q Nat Commun. 2022; 13(1):7838.

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Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase from .

Roca C, Avalos-Padilla Y, Prieto-Simon B, Iglesias V, Ramirez M, Imperial S Pharmaceutics. 2022; 14(11).

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