Fimbriation and Curliation in Escherichia Coli O157:H7: a Paradigm of Intestinal and Environmental Colonization

Overview

Journal Gut Microbes

Specialties Gastroenterology
Microbiology

Date 2012 May 23

PMID 22614704

Citations 19

Authors

Sonja J Lloyd

Jennifer M Ritchie

Alfredo G Torres

Affiliations

Soon will be listed here.

Abstract

Shiga toxin-producing Escherichia coli (STEC) serotypes, particularly E. coli O157:H7, possess a variety of fimbrial and afimbrial adhesins which have emerged as important contributors to intestinal colonization. E. coli O157:H7 possesses two chromosomal operons encoding long polar fimbriae (Lpf), which have been found to influence adherence in vitro and colonization in vivo. In a recent Infection and Immunity paper, we further explored the role of Lpf in E. coli O157:H7 intestinal colonization by using the infant rabbit model of STEC infection. We found that an E. coli O157:H7 Lpf-deficient mutant was outcompeted in the rabbit intestine by its parental strain, which may suggest that Lpf contributes to colonization. In contrast, the Lpf-deficient mutant showed an increased adherence to cultured intestinal epithelial cells, and we discovered that this strain overexpressed curli fibers. In this addendum article, we provide a continued perspective on the predicted roles of Lpf and curli, both in vivo and in vitro.

Citing Articles

Lipopolysaccharide Core Truncation in Invasive O157:H7 ATCC 43895 Impairs Flagella and Curli Biosynthesis and Reduces Cell Invasion Ability.

Sheng H, Ndeddy Aka R, Wu S Int J Mol Sci. 2024; 25(17).

PMID: 39273173 PMC: 11394844. DOI: 10.3390/ijms25179224.

Optimization of Multivalent Gold Nanoparticle Vaccines Eliciting Humoral and Cellular Immunity in an Model of Enterohemorrhagic Escherichia coli O157:H7 Colonization.

Sanchez-Villamil J, Tapia D, Torres A mSphere. 2022; 7(1):e0093421.

PMID: 35044806 PMC: 8769200. DOI: 10.1128/msphere.00934-21.

O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle.

Sheng H, Xue Y, Zhao W, Hovde C, Minnich S Microorganisms. 2020; 8(4).

PMID: 32316415 PMC: 7232329. DOI: 10.3390/microorganisms8040580.

Expression and Functional Characterization of Various Chaperon-Usher Fimbriae, Curli Fimbriae, and Type 4 Pili of Enterohemorrhagic O157:H7 Sakai.

Elpers L, Hensel M Front Microbiol. 2020; 11:378.

PMID: 32265855 PMC: 7098969. DOI: 10.3389/fmicb.2020.00378.

Adherence Factor Expression in -Like Conditions.

Chanin R, Nickerson K, Llanos-Chea A, Sistrunk J, Rasko D, Kumar D mSphere. 2019; 4(6).

PMID: 31722995 PMC: 6854044. DOI: 10.1128/mSphere.00751-19.

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