Distinct Functions of Regions 1.1 and 1.2 of RNA Polymerase σ Subunits from Escherichia Coli and Thermus Aquaticus in Transcription Initiation

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2012 May 19

PMID 22605342

Citations 8

Authors

Nataliya Miropolskaya

Artem Ignatov

Irina Bass

Ekaterina Zhilina

Danil Pupov

Andrey Kulbachinskiy

Affiliations

Soon will be listed here.

Abstract

RNA polymerase (RNAP) from thermophilic Thermus aquaticus is characterized by higher temperature of promoter opening, lower promoter complex stability, and higher promoter escape efficiency than RNAP from mesophilic Escherichia coli. We demonstrate that these differences are in part explained by differences in the structures of the N-terminal regions 1.1 and 1.2 of the E. coli σ(70) and T. aquaticus σ(A) subunits. In particular, region 1.1 and, to a lesser extent, region 1.2 of the E. coli σ(70) subunit determine higher promoter complex stability of E. coli RNAP. On the other hand, nonconserved amino acid substitutions in region 1.2, but not region 1.1, contribute to the differences in promoter opening between E. coli and T. aquaticus RNAPs, likely through affecting the σ subunit contacts with DNA nucleotides downstream of the -10 element. At the same time, substitutions in σ regions 1.1 and 1.2 do not affect promoter escape by E. coli and T. aquaticus RNAPs. Thus, evolutionary substitutions in various regions of the σ subunit modulate different steps of the open promoter complex formation pathway, with regions 1.1 and 1.2 affecting promoter complex stability and region 1.2 involved in DNA melting during initiation.

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