Adipose-derived Stem Cells on the Healing of Ischemic Colitis: a Therapeutic Effect by Angiogenesis

Overview

Journal Int J Colorectal Dis

Specialties Gastroenterology
General Surgery

Date 2012 May 17

PMID 22588671

Citations 5

Authors

Hyun Ho Joo

Hye Jung Jo

Tae Doo Jung

Min Sung Ahn

Ki Beom Bae

Kwan Hee Hong

Jeong Kim

Jong Tae Kim

Sun Hee Kim

Young Il Yang

Affiliations

Soon will be listed here.

Abstract

Background: There has been no specific treatment for ischemic colitis. We verified the effects of adipose-derived stem cells (ASCs) on ischemia-induced colitis in a rat model.

Methods: Forty male Sprague-Dawley rats (10 weeks old; weight, 350 ± 20 g) were divided into two groups: a control group (only fibrinogen and thrombin injected, n = 20) and an ASC group (local implantation of ASCs mixed with thrombin and fibrinogen, n = 20). An ischemic colitis model was established by modifying Nagahata's methods with double-blind randomization. ASCs (1 × 10(6) cells) were implanted intramurally into the ischemic area using a fibrin glue mixture. The severity of adhesion, degree of ileus, the number and size of the ulcers, Wallace macroscopic and microscopic scores, and microvascular density were measured.

Results: The degree of ileus was significantly lower, and significantly fewer and smaller ulcerations were found in the ASC group than those in the control group. Wallace macroscopic and microscopic scores were lower in the ASC group than in the control group (1.90 ± 1.22 versus 3.25 ± 1.83, p < 0.01 and 1.55 ± 1.88 versus 2.84 ± 1.89, p < 0.05, respectively). Microvascular density was higher in the ASC group than in the control (54.45 ± 19.45 versus 26.54 ± 13.14, p < 0.01, respectively).

Conclusions: Local implantation of ASCs into an ischemic-injured colonic wall reduced the grade of ischemic injury and enhanced tissue healing by promoting angiogenesis.

Citing Articles

Local Application of Minimally Manipulated Autologous Stromal Vascular Fraction (SVF) Reduces Inflammation and Improves Bilio-Biliary Anastomosis Integrity.

Klabukov I, Shatveryan G, Bagmet N, Aleshina O, Ivanova E, Savina V Int J Mol Sci. 2025; 26(1.

PMID: 39796076 PMC: 11720677. DOI: 10.3390/ijms26010222.

The Proliferation and Differentiation of Adipose-Derived Stem Cells in Neovascularization and Angiogenesis.

Hutchings G, Janowicz K, Moncrieff L, Dompe C, Strauss E, Kocherova I Int J Mol Sci. 2020; 21(11).

PMID: 32471255 PMC: 7312564. DOI: 10.3390/ijms21113790.

Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets.

Hara T, Soyama A, Adachi T, Kobayashi S, Sakai Y, Maruya Y Regen Ther. 2020; 14:79-86.

PMID: 31988997 PMC: 6970135. DOI: 10.1016/j.reth.2019.11.001.

Therapeutic angiogenesis of adipose-derived stem cells for ischemic diseases.

Zhao L, Johnson T, Liu D Stem Cell Res Ther. 2017; 8(1):125.

PMID: 28583178 PMC: 5460534. DOI: 10.1186/s13287-017-0578-2.

Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh.

Deng M, Gu Y, Liu Z, Qi Y, Ma G, Kang N Stem Cells Int. 2015; 2015:350718.

PMID: 26106426 PMC: 4464689. DOI: 10.1155/2015/350718.

References

Gonzalez-Rey E, Anderson P, Gonzalez M, Rico L, Buscher D, Delgado M . Human adult stem cells derived from adipose tissue protect against experimental colitis and sepsis. Gut. 2009; 58(7):929-39. DOI: 10.1136/gut.2008.168534. View

Huguier M, Barrier A, Boelle P, Houry S, Lacaine F . Ischemic colitis. Am J Surg. 2006; 192(5):679-84. DOI: 10.1016/j.amjsurg.2005.09.018. View

Kang S, Lee D, Bae Y, Kim H, Baik S, Jung J . Improvement of neurological deficits by intracerebral transplantation of human adipose tissue-derived stromal cells after cerebral ischemia in rats. Exp Neurol. 2003; 183(2):355-66. DOI: 10.1016/s0014-4886(03)00089-x. View

Tadauchi A, Narita Y, Kagami H, Niwa Y, Ueda M, Goto H . Novel cell-based therapeutic strategy for ischemic colitis with use of bone marrow-derived mononuclear cells in rats. Dis Colon Rectum. 2009; 52(8):1443-51. DOI: 10.1007/DCR.0b013e3181a79549. View

Sreenarasimhaiah J . Diagnosis and management of intestinal ischaemic disorders. BMJ. 2003; 326(7403):1372-6. PMC: 1126251. DOI: 10.1136/bmj.326.7403.1372. View

Higgins P, Davis K, Laine L . Systematic review: the epidemiology of ischaemic colitis. Aliment Pharmacol Ther. 2004; 19(7):729-38. DOI: 10.1111/j.1365-2036.2004.01903.x. View

Khalil P, Weiler V, Nelson P, Khalil M, Moosmann S, Mutschler W . Nonmyeloablative stem cell therapy enhances microcirculation and tissue regeneration in murine inflammatory bowel disease. Gastroenterology. 2007; 132(3):944-54. DOI: 10.1053/j.gastro.2006.12.029. View

Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove C, Bovenkerk J . Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004; 109(10):1292-8. DOI: 10.1161/01.CIR.0000121425.42966.F1. View

Gonzalez M, Gonzalez-Rey E, Rico L, Buscher D, Delgado M . Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Gastroenterology. 2009; 136(3):978-89. DOI: 10.1053/j.gastro.2008.11.041. View

10.

Mizuno H . Adipose-derived stem cells for tissue repair and regeneration: ten years of research and a literature review. J Nippon Med Sch. 2009; 76(2):56-66. DOI: 10.1272/jnms.76.56. View

11.

Nakagami H, Maeda K, Morishita R, Iguchi S, Nishikawa T, Takami Y . Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue-derived stromal cells. Arterioscler Thromb Vasc Biol. 2005; 25(12):2542-7. DOI: 10.1161/01.ATV.0000190701.92007.6d. View

12.

Gimble J, Guilak F . Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003; 5(5):362-9. DOI: 10.1080/14653240310003026. View

13.

Matsubara H, Murohara T, Ikeda U, Shintani S, Masaki H, Amano K . Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial. Lancet. 2002; 360(9331):427-35. DOI: 10.1016/S0140-6736(02)09670-8. View

14.

BOLEY S, Schwartz S, Lash J, STERNHILL V . Reversible vascular occlusion of the colon. Surg Gynecol Obstet. 1963; 116:53-60. View

15.

Zeng Q, Li X, Beck G, Balian G, Shen F . Growth and differentiation factor-5 (GDF-5) stimulates osteogenic differentiation and increases vascular endothelial growth factor (VEGF) levels in fat-derived stromal cells in vitro. Bone. 2006; 40(2):374-81. DOI: 10.1016/j.bone.2006.09.022. View

16.

Ando Y, Inaba M, Sakaguchi Y, Tsuda M, Quan G, Omae M . Subcutaneous adipose tissue-derived stem cells facilitate colonic mucosal recovery from 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. Inflamm Bowel Dis. 2008; 14(6):826-38. DOI: 10.1002/ibd.20382. View

17.

Nagahata Y, Azumi Y, Akimoto T, Nomura H, Ichihara T, Idei H . Role of platelet activating factor on severity of ischemic colitis. Dis Colon Rectum. 1999; 42(2):218-24. DOI: 10.1007/BF02237132. View

18.

Lu F, Mizuno H, Uysal C, Cai X, Ogawa R, Hyakusoku H . Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg. 2008; 121(1):50-58. DOI: 10.1097/01.prs.0000293876.10700.b8. View

19.

Cao Y, Sun Z, Liao L, Meng Y, Han Q, Zhao R . Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun. 2005; 332(2):370-9. DOI: 10.1016/j.bbrc.2005.04.135. View

20.

Kondo K, Shintani S, Shibata R, Murakami H, Murakami R, Imaizumi M . Implantation of adipose-derived regenerative cells enhances ischemia-induced angiogenesis. Arterioscler Thromb Vasc Biol. 2008; 29(1):61-6. DOI: 10.1161/ATVBAHA.108.166496. View