If You Don't Look, You Won't See: Intravital Multiphoton Imaging of Primary and Metastatic Breast Cancer

Overview

Journal J Mammary Gland Biol Neoplasia

Date 2012 May 15

PMID 22581273

Citations 9

Authors

Laura Bonapace

Jeffrey Wyckoff

Thomas Oertner

Jacco van Rheenen

Tobias Junt

Mohamed Bentires-Alj

Affiliations

Soon will be listed here.

Abstract

A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.

Citing Articles

Assessment of MRI to estimate metastatic dissemination risk and prometastatic effects of chemotherapy.

Karagiannis G, Bianchi A, Sanchez L, Ambadipudi K, Cui M, Anampa J NPJ Breast Cancer. 2022; 8(1):101.

PMID: 36056005 PMC: 9440218. DOI: 10.1038/s41523-022-00463-5.

Present Application and Perspectives of Organoid Imaging Technology.

Fei K, Zhang J, Yuan J, Xiao P Bioengineering (Basel). 2022; 9(3).

PMID: 35324810 PMC: 8945799. DOI: 10.3390/bioengineering9030121.

Real-time, high-resolution imaging of tumor cells in genetically engineered and orthotopic models of thyroid cancer.

Shanja-Grabarz X, Coste A, Entenberg D, Di Cristofano A Endocr Relat Cancer. 2020; 27(10):529-539.

PMID: 32698130 PMC: 7450603. DOI: 10.1530/ERC-20-0295.

SYK inhibition blocks proliferation and migration of glioma cells and modifies the tumor microenvironment.

Moncayo G, Grzmil M, Smirnova T, Zmarz P, Huber R, Hynx D Neuro Oncol. 2018; 20(5):621-631.

PMID: 29401256 PMC: 5892144. DOI: 10.1093/neuonc/noy008.

Review of quantitative multiscale imaging of breast cancer.

Pinkert M, Salkowski L, Keely P, Hall T, Block W, Eliceiri K J Med Imaging (Bellingham). 2018; 5(1):010901.

PMID: 29392158 PMC: 5777512. DOI: 10.1117/1.JMI.5.1.010901.

References

Lohela M, Werb Z . Intravital imaging of stromal cell dynamics in tumors. Curr Opin Genet Dev. 2009; 20(1):72-8. PMC: 2821950. DOI: 10.1016/j.gde.2009.10.011. View

Eggeling C, Volkmer A, Seidel C . Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy. Chemphyschem. 2005; 6(5):791-804. DOI: 10.1002/cphc.200400509. View

Ahmed F, Wyckoff J, Lin E, Wang W, Wang Y, Hennighausen L . GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice. Cancer Res. 2002; 62(24):7166-9. View

Sipkins D, Wei X, Wu J, Runnels J, Cote D, Means T . In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature. 2005; 435(7044):969-73. PMC: 2570168. DOI: 10.1038/nature03703. View

Wyckoff J, Jones J, Condeelis J, Segall J . A critical step in metastasis: in vivo analysis of intravasation at the primary tumor. Cancer Res. 2000; 60(9):2504-11. View

Valastyan S, Weinberg R . Tumor metastasis: molecular insights and evolving paradigms. Cell. 2011; 147(2):275-92. PMC: 3261217. DOI: 10.1016/j.cell.2011.09.024. View

Mahmood U, Tung C, Bogdanov Jr A, Weissleder R . Near-infrared optical imaging of protease activity for tumor detection. Radiology. 1999; 213(3):866-70. DOI: 10.1148/radiology.213.3.r99dc14866. View

Alexander S, Koehl G, Hirschberg M, Geissler E, Friedl P . Dynamic imaging of cancer growth and invasion: a modified skin-fold chamber model. Histochem Cell Biol. 2008; 130(6):1147-54. DOI: 10.1007/s00418-008-0529-1. View

Sahai E . Illuminating the metastatic process. Nat Rev Cancer. 2007; 7(10):737-49. DOI: 10.1038/nrc2229. View

10.

Zomer A, Beerling E, Vlug E, van Rheenen J . Real-time intravital imaging of cancer models. Clin Transl Oncol. 2011; 13(12):848-54. DOI: 10.1007/s12094-011-0745-3. View

11.

Giampieri S, Manning C, Hooper S, Jones L, Hill C, Sahai E . Localized and reversible TGFbeta signalling switches breast cancer cells from cohesive to single cell motility. Nat Cell Biol. 2009; 11(11):1287-96. PMC: 2773241. DOI: 10.1038/ncb1973. View

12.

Egeblad M, Ewald A, Askautrud H, Truitt M, Welm B, Bainbridge E . Visualizing stromal cell dynamics in different tumor microenvironments by spinning disk confocal microscopy. Dis Model Mech. 2008; 1(2-3):155-67. PMC: 2562195. DOI: 10.1242/dmm.000596. View

13.

Kreisel D, Nava R, Li W, Zinselmeyer B, Wang B, Lai J . In vivo two-photon imaging reveals monocyte-dependent neutrophil extravasation during pulmonary inflammation. Proc Natl Acad Sci U S A. 2010; 107(42):18073-8. PMC: 2964224. DOI: 10.1073/pnas.1008737107. View

14.

Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha V, Condeelis J, Segall J . Intravital imaging of metastatic behavior through a mammary imaging window. Nat Methods. 2008; 5(12):1019-21. PMC: 2820719. DOI: 10.1038/nmeth.1269. View

15.

Yang M, Baranov E, Wang J, Jiang P, Wang X, Sun F . Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc Natl Acad Sci U S A. 2002; 99(6):3824-9. PMC: 122608. DOI: 10.1073/pnas.052029099. View

16.

Helmchen F, Denk W . Deep tissue two-photon microscopy. Nat Methods. 2005; 2(12):932-40. DOI: 10.1038/nmeth818. View

17.

Pittet M, Weissleder R . Intravital imaging. Cell. 2011; 147(5):983-91. PMC: 3824153. DOI: 10.1016/j.cell.2011.11.004. View

18.

Borowsky A . Choosing a mouse model: experimental biology in context--the utility and limitations of mouse models of breast cancer. Cold Spring Harb Perspect Biol. 2011; 3(9):a009670. PMC: 3181037. DOI: 10.1101/cshperspect.a009670. View

19.

Fidler I . The pathogenesis of cancer metastasis: the 'seed and soil' hypothesis revisited. Nat Rev Cancer. 2003; 3(6):453-8. DOI: 10.1038/nrc1098. View

20.

Condeelis J, Weissleder R . In vivo imaging in cancer. Cold Spring Harb Perspect Biol. 2010; 2(12):a003848. PMC: 2982169. DOI: 10.1101/cshperspect.a003848. View