Real-time Intravital Imaging of Cancer Models

Overview

Journal Clin Transl Oncol

Specialty Oncology

Date 2011 Dec 1

PMID 22126727

Citations 4

Authors

A Zomer

E Beerling

E J Vlug

J van Rheenen

Affiliations

Soon will be listed here.

Abstract

High-resolution intravital imaging (IVM) has proven to be a powerful technique to visualise dynamic processes that are important for tumour progression, such as the interplay between tumour cells and cellular components of the tumour microenvironment. The development of IVM tools, including imaging windows and photo-marking of individual cells, has led to the visualisation of dynamic processes and tracking of individual cells over a time span of days. In order to visualise these dynamic processes, several strategies have been described to develop fluorescent IVM tumour models. Genetic tools to engineer fluorescent tumour cell lines have advanced the applications of cell line-based tumour models to study, for example, changes in behaviour or transcriptional and differentiation state of individual cells in a tumour. In order to study tumour progression, fluorescent genetic mouse models have been engineered that better recapitulate human tumours. These technically challenging tumour models are key in visualising dynamic processes during cancer progression and in the translational aspects of IVM experiments.

Citing Articles

Imaging windows for long-term intravital imaging: General overview and technical insights.

Alieva M, Ritsma L, Giedt R, Weissleder R, van Rheenen J Intravital. 2017; 3(2):e29917.

PMID: 28243510 PMC: 5312719. DOI: 10.4161/intv.29917.

Tyrosine phosphatase SHP2 increases cell motility in triple-negative breast cancer through the activation of SRC-family kinases.

Sausgruber N, Coissieux M, Britschgi A, Wyckoff J, Aceto N, Leroy C Oncogene. 2014; 34(17):2272-8.

PMID: 24931162 DOI: 10.1038/onc.2014.170.

Intravital FRET imaging of tumor cell viability and mitosis during chemotherapy.

Janssen A, Beerling E, Medema R, van Rheenen J PLoS One. 2013; 8(5):e64029.

PMID: 23691140 PMC: 3654962. DOI: 10.1371/journal.pone.0064029.

If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer.

Bonapace L, Wyckoff J, Oertner T, van Rheenen J, Junt T, Bentires-Alj M J Mammary Gland Biol Neoplasia. 2012; 17(2):125-9.

PMID: 22581273 DOI: 10.1007/s10911-012-9250-8.

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