Inhibition of Human Platelet Activation by Polymorphonuclear Leukocytes

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1990 Oct 1

PMID 2257433

Citations 5

Authors

M A Schattner

J R Geffner

M A Isturiz

M A Lazzari

Affiliations

Soon will be listed here.

Abstract

1. The effect of unstimulated human polymorphonuclear leukocytes (PMNs) on platelet activation was examined. 2. Human platelet aggregation and adenosine 5'-triphosphate (ATP) release induced by collagen (1-2 micrograms ml-1); thrombin (0.01-0.02 u ml-1) or arachidonic acid (AA) (0.1-0.2 mM) were markedly inhibited when conducted in the presence of unstimulated PMNs. 3. Platelet inhibition induced by PMNs was dependent on the number of PMNs and on the incubation time of the mixed cell suspension. 4. Platelet inhibition was not reversed in time when PMNs were depleted from the mixed-cell suspension. 5. PMN-mediated platelet-inhibition was not mediated by AA metabolites, oxygen reactive intermediates, nitric oxide or proteases. 6. The factor(s) accounting for the platelet inhibition mediated by PMNs are not yet characterized.

Citing Articles

Vipegitide: a folded peptidomimetic partial antagonist of α2β1 integrin with antiplatelet aggregation activity.

Momic T, Katzhendler J, Shai E, Noy E, Senderowitz H, Eble J Drug Des Devel Ther. 2015; 9:291-304.

PMID: 25609915 PMC: 4294129. DOI: 10.2147/DDDT.S72844.

Nitrite is produced by elicited but not by circulating neutrophils.

Stewart A, Dusting G, Giarracca R, Harris T, Lim Y, Sobey C Mediators Inflamm. 1993; 2(5):349-56.

PMID: 18475544 PMC: 2365423. DOI: 10.1155/S0962935193000481.

Bidirectional modulation of platelet and polymorphonuclear leukocyte activities.

Del Maschio A, Dejana E, Bazzoni G Ann Hematol. 1993; 67(1):23-31.

PMID: 8334196 DOI: 10.1007/BF01709662.

Downregulation of human platelet reactivity by neutrophils. Participation of lipoxygenase derivatives and adhesive proteins.

Valles J, Santos M, Marcus A, Safier L, Broekman M, Islam N J Clin Invest. 1993; 92(3):1357-65.

PMID: 7690778 PMC: 288277. DOI: 10.1172/JCI116709.

Platelet-activating factor: the effector of protein-rich plasma extravasation and nitric oxide synthase induction in rat immune complex peritonitis.

Steil A, Garcia Rodriguez M, Alonso A, Crespo M, Bosca L Br J Pharmacol. 1995; 114(4):895-901.

PMID: 7539698 PMC: 1510210. DOI: 10.1111/j.1476-5381.1995.tb13288.x.

References

Gryglewski R, Palmer R, Moncada S . Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor. Nature. 1986; 320(6061):454-6. DOI: 10.1038/320454a0. View

Rhee B, Hall E, McIntire L . Platelet modulation of polymorphonuclear leukocyte shear induced aggregation. Blood. 1986; 67(1):240-6. View

Tringale S, Tauber A . Identification of distinct activation pathways of the human neutrophil NADPH-oxidase. J Immunol. 1986; 137(9):2925-9. View

Chignard M, Selak M, Smith J . Direct evidence for the existence of a neutrophil-derived platelet activator (neutrophilin). Proc Natl Acad Sci U S A. 1986; 83(22):8609-13. PMC: 386980. DOI: 10.1073/pnas.83.22.8609. View

Palmer R, Ferrige A, Moncada S . Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 1987; 327(6122):524-6. DOI: 10.1038/327524a0. View

Geffner J, Giordano M, Palermo M, Prat A, Serebrinsky G, Isturiz M . Neutrophil-mediated cytotoxicity triggered by immune complexes: the role of reactive oxygen metabolites. Clin Exp Immunol. 1987; 69(3):668-75. PMC: 1542387. View

Rimele T, Sturm R, Adams L, Henry D, Heaslip R, Weichman B . Interaction of neutrophils with vascular smooth muscle: identification of a neutrophil-derived relaxing factor. J Pharmacol Exp Ther. 1988; 245(1):102-11. View

Moncada S, Palmer R, Higgs E . The discovery of nitric oxide as the endogenous nitrovasodilator. Hypertension. 1988; 12(4):365-72. DOI: 10.1161/01.hyp.12.4.365. View

Turner S, Tainer J, Lynn W . Biogenesis of chemotactic molecules by the arachidonate lipoxygenase system of platelets. Nature. 1975; 257(5528):680-1. DOI: 10.1038/257680a0. View

10.

Levine P, Weinger R, Simon J, Scoon K, Krinsky N . Leukocyte-platelet interaction. Release of hydrogen peroxide by granulocytes as a modulator of platelet reactions. J Clin Invest. 1976; 57(4):955-63. PMC: 436739. DOI: 10.1172/JCI108372. View

11.

UBATUBA F, Moncada S, Vane J . The effect of prostacyclin (PGT2) on platelet behaviour. Thrombus formation in vivo and bleeding time. Thromb Haemost. 1979; 41(2):425-35. View

12.

Kitagawa S, Takaku F, Sakamoto S . Evidence that proteases are involved in superoxide production by human polymorphonuclear leukocytes and monocytes. J Clin Invest. 1980; 65(1):74-81. PMC: 371341. DOI: 10.1172/JCI109662. View

13.

Lotner G, Lynch J, Betz S, Henson P . Human neutrophil-derived platelet activating factor. J Immunol. 1980; 124(2):676-84. View

14.

Marcus A, Safier L, ULLMAN H, Wong K, Broekman M, Weksler B . Effects of acetyl glyceryl ether phosphorylcholine on human platelet function in vitro. Blood. 1981; 58(5):1027-31. View

15.

Blackwell G, Flower R . Inhibition of phospholipase. Br Med Bull. 1983; 39(3):260-4. DOI: 10.1093/oxfordjournals.bmb.a071830. View

16.

Martin W, Villani G, Jothianandan D, FURCHGOTT R . Blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation of rabbit aorta by certain ferrous hemoproteins. J Pharmacol Exp Ther. 1985; 233(3):679-85. View

17.

Zoucas E, Faldt R, Ankerst J . Effect of latex-stimulated granulocytes on platelet aggregation in man. Haemostasis. 1985; 15(3):176-81. DOI: 10.1159/000215141. View

18.

Mehta P, Mehta J, Lawson D, Krop I, Letts L . Leukotrienes potentiate the effects of epinephrine and thrombin on human platelet aggregation. Thromb Res. 1986; 41(5):731-8. DOI: 10.1016/0049-3848(86)90370-1. View