Nanog: a Potential Biomarker for Liver Metastasis of Colorectal Cancer

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2012 May 8

PMID 22562535

Citations 29

Authors

Feng Xu

Chaoliu Dai

Rui Zhang

Yang Zhao

Songlin Peng

Changjun Jia

Affiliations

Soon will be listed here.

Abstract

Background: At present, the relationship between Nanog expression and the biological behavior and prognosis of colorectal cancer is still unclear.

Aim: The purpose of this study was to evaluate the expression and regulatory effects of Nanog in colorectal cancer and the correlation between Nanog protein expression and the prognosis of patients with colorectal cancer.

Materials And Methods: The differential expression of genes between CD133+ tumor cells and CD133- tumor cells were detected using RT(2) Profiler™ PCR Array. The Nanog mRNA expression level was detected by RT-PCR and the protein level was detected using immunohistochemistry staining. The relationship between Nanog expression and clinicopathological parameters of colorectal cancer was determined.

Results: Nanog were expressed significantly higher in CD133+ tumor cells compared to CD133- tumor cells. It was observed that 72 (20.00 %) of the 360 cases positively expressed Nanog. Univariate analyses indicated that Nanog expression was related to histological grade, lymph node metastasis, TNM stage, and liver metastasis (P = 0.005, 0.001, 0.001 and 0.012, respectively). Spearman correlation analysis showed that Nanog expression has a linear correlation to liver metastasis (P = 0.001). After conducting multivariate analysis, histological grade, TNM stage, and Nanog were found to be related to liver metastasis (P = 0.020, 0.01 and 0.001, respectively). In the Cox regression test, the histological grade, Lymph node metastasis, TNM stage, liver metastasis, and Nanog were detected as the independent prognostic factors (P = 0.02, 0.045, 0.01, 0.001 and 0.001, respectively).

Conclusions: Nanog protein may be a potential biomarker for postoperative liver metastasis of colorectal cancer.

Citing Articles

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