Immunological Responses and Actin Dynamics in Macrophages Are Controlled by N-cofilin but Are Independent from ADF

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 May 5

PMID 22558315

Citations 16

Authors

Friederike Jonsson

Christine B Gurniak

Bernhard Fleischer

Gregor Kirfel

Walter Witke

Affiliations

Soon will be listed here.

Abstract

Dynamic changes in the actin cytoskeleton are essential for immune cell function and a number of immune deficiencies have been linked to mutations, which disturb the actin cytoskeleton. In macrophages and dendritic cells, actin remodelling is critical for motility, phagocytosis and antigen presentation, however the actin binding proteins, which control antigen presentation have been poorly characterized. Here we dissect the specific roles of the family of ADF/cofilin F-actin depolymerizing factors in macrophages and in local immune responses. Macrophage migration, cell polarization and antigen presentation to T-cells require n-cofilin mediated F-actin remodelling. Using a conditional mouse model, we show that n-cofilin also controls MHC class II-dependent antigen presentation. Other cellular processes such as phagocytosis and antigen processing were found to be independent of n-cofilin. Our data identify n-cofilin as a novel regulator of antigen presentation, while ADF on the other hand is dispensable for macrophage motility and antigen presentation.

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