Simian Virus 40-transformed Cells Express New Species of Proteins Precipitable by Anti-simian Virus 40 Tumor Serum

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1979 Aug 1

PMID 225566

Citations 140

Authors

M Kress

E May

R CASSINGENA

P May

Affiliations

Soon will be listed here.

Abstract

In addition to the virus-coded large-T and small-t antigens, two new classes of proteins were immunoprecipitated by anti-simian virus 40 (SV40) tumor serum from extracts of various SV40-transformed cell lines. These were as follows: (i) proteins (termed "super-T proteins") with an Mr higher than that of large-T antigen (86,000), which were found in many SV40-transformed cell lines derived from mouse and rat cells (super-T proteins and large-T antigen appeared to have closely related structures as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides); (ii) proteins (termed "55K proteins") with an Mr ranging from 50,000 to 60,000, which were present in all SV40-transformed cell lines examined so far, including those obtained by chromosome-mediated gene transfer. The 55K proteins were not structurally related to large-T antigens, as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides. Our data are compatible with the assumption that the 55K proteins are largely or totally cell coded.

Citing Articles

Effects of Tp53 Gene Mutations on the Survival of Non-Small Cell Lung Cancer (NSCLC); A Short Review.

Zhang C, Yang C, Shi Q Cancer Manag Res. 2025; 17():65-82.

PMID: 39830995 PMC: 11742633. DOI: 10.2147/CMAR.S495006.

Role of artificial intelligence in cancer detection using protein p53: A Review.

Patil M, Bihari A Mol Biol Rep. 2024; 52(1):46.

PMID: 39658610 DOI: 10.1007/s11033-024-10051-4.

p53 tumor suppressor directly activates conserved asymmetric stem cell division regulators.

Manzanero-Ortiz S, Franco M, Laxmeesha M, Carmena A iScience. 2024; 27(11):111118.

PMID: 39524346 PMC: 11546965. DOI: 10.1016/j.isci.2024.111118.

Impacts of cytoplasmic p53 aggregates on the prognosis and the transcriptome in lung squamous cell carcinoma.

Nishitsuji K, Mito R, Ikezaki M, Yano H, Fujiwara Y, Matsubara E Cancer Sci. 2024; 115(9):2947-2960.

PMID: 39031627 PMC: 11462941. DOI: 10.1111/cas.16252.

Understanding the complexity of p53 in a new era of tumor suppression.

Liu Y, Su Z, Tavana O, Gu W Cancer Cell. 2024; 42(6):946-967.

PMID: 38729160 PMC: 11190820. DOI: 10.1016/j.ccell.2024.04.009.

References

Girardi A, JENSEN F, KOPROWSKI H . SV40-INDUCED TRANFORMATION OF HUMAN DIPLOID CELLS: CRISIS AND RECOVERY. J Cell Comp Physiol. 1965; 65:69-83. DOI: 10.1002/jcp.1030650110. View

Bose K, Karran P, Strauss B . Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts. Proc Natl Acad Sci U S A. 1978; 75(2):794-8. PMC: 411343. DOI: 10.1073/pnas.75.2.794. View

Paucha E, Harvey R, Smith A . Cell-free synthesis of simian virus 40 T-antigens. J Virol. 1978; 28(1):154-70. PMC: 354255. DOI: 10.1128/JVI.28.1.154-170.1978. View

Chang C, Simmons D, Martin M, Mora P . Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells. J Virol. 1979; 31(2):463-71. PMC: 353469. DOI: 10.1128/JVI.31.2.463-471.1979. View

Melero J, Stitt D, Mangel W, Carroll R . Identification of new polypeptide species (48-55K) immunoprecipitable by antiserum to purified large T antigen and present in SV40-infected and -transformed cells. Virology. 1979; 93(2):466-80. DOI: 10.1016/0042-6822(79)90250-2. View

Smith A, Smith R, Paucha E . Extraction and fingerprint analysis of simian virus 40 large and small T-antigens. J Virol. 1978; 28(1):140-53. PMC: 354254. DOI: 10.1128/JVI.28.1.140-153.1978. View

Rassoulzadegan M, Perbal B, Cuzin F . Growth control in simian virus 40-transformed rat cells: temperature-independent expression of the transformed phenotype in tsA transformants derived by agar selection. J Virol. 1978; 28(1):1-5. PMC: 354240. DOI: 10.1128/JVI.28.1.1-5.1978. View

Lane D, CRAWFORD L . T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979; 278(5701):261-3. DOI: 10.1038/278261a0. View

Edwards C, Khoury G, Martin R . Phosphorylation of T-antigen and control T-antigen expression in cells transformed by wild-type and tsA mutants of simian virus 40. J Virol. 1979; 29(2):753-62. PMC: 353207. DOI: 10.1128/JVI.29.2.753-762.1979. View

10.

CASSINGENA R, Suarez H, Lavialle C, Persuy M, Ermonval M . Transformation of normal diploid cells by isolated metaphase chromosomes of virus-transformed or spontaneous tumor cells. Gene. 1978; 4(4):337-49. DOI: 10.1016/0378-1119(78)90050-1. View

11.

Smart J, Ito Y . Three species of polyoma virus tumor antigens share common peptides probably near the amino termini of the proteins. Cell. 1978; 15(4):1427-37. DOI: 10.1016/0092-8674(78)90066-1. View

12.

Hutchinson M, Hunter T, Eckhart W . Characterization of T antigens in polyoma-infected and transformed cells. Cell. 1978; 15(1):65-77. DOI: 10.1016/0092-8674(78)90083-1. View

13.

Feunteun J, Kress M, Gardes M, Monier R . Viable deletion mutants in the simian virus 40 early region. Proc Natl Acad Sci U S A. 1978; 75(9):4455-9. PMC: 336134. DOI: 10.1073/pnas.75.9.4455. View

14.

May E, Kress M, May P . Characterization of two SV40 early mRNAs and evidence for a nuclear "prespliced" RNA species. Nucleic Acids Res. 1978; 5(9):3083-99. PMC: 342233. DOI: 10.1093/nar/5.9.3083. View

15.

Gaudray P, Rassoulzadegan M, Cuzin F . Expression of simian virus 40 early genes in transformed rat cells is correlated with maintenance of the transformed phenotype. Proc Natl Acad Sci U S A. 1978; 75(10):4987-91. PMC: 336247. DOI: 10.1073/pnas.75.10.4987. View

16.

BOUCK N, Beales N, Shenk T, Berg P, DI MAYORCA G . New region of the simian virus 40 genome required for efficient viral transformation. Proc Natl Acad Sci U S A. 1978; 75(5):2473-7. PMC: 392576. DOI: 10.1073/pnas.75.5.2473. View

17.

CRAWFORD L, Cole C, Smith A, Paucha E, Tegtmeyer P, Rundell K . Organization and expression of early genes of simian virus 40. Proc Natl Acad Sci U S A. 1978; 75(1):117-21. PMC: 411195. DOI: 10.1073/pnas.75.1.117. View

18.

Fiers W, Contreras R, Haegemann G, Rogiers R, Van de Voorde A, Van Heuverswyn H . Complete nucleotide sequence of SV40 DNA. Nature. 1978; 273(5658):113-20. DOI: 10.1038/273113a0. View

19.

Brockman W . Transformation of BALB/c-3T3 cells by tsA mutants of simian virus 40: temperature sensitivity of the transformed phenotype and retransofrmation by wild-type virus. J Virol. 1978; 25(3):860-70. PMC: 525980. DOI: 10.1128/JVI.25.3.860-870.1978. View

20.

Berk A, Sharp P . Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978; 75(3):1274-8. PMC: 411453. DOI: 10.1073/pnas.75.3.1274. View