Chemical Reporters for Monitoring RNA Synthesis and Poly(A) Tail Dynamics

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2012 Apr 20

PMID 22513998

Citations 28

Authors

Markus Grammel

Howard Hang

Nicholas K Conrad

Affiliations

Soon will be listed here.

Abstract

A versatile "clickable" nucleoside: Metabolic labeling of cells is useful in studying the dynamics of biological molecules. N(6) pA can be utilized by all three mammalian RNA polymerases, as well as poly(A) polymerase. Because of its alkyne modification, RNA labeled with N(6) pA can be visualized and purified by using click chemistry.

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