High-throughput Screening for Streptomyces Antibiotic Biosynthesis Activators

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2012 Apr 17

PMID 22504805

Citations 6

Authors

Li Chen

Yemin Wang

Hang Guo

Min Xu

Zixin Deng

Meifeng Tao

Affiliations

Soon will be listed here.

Abstract

A genomic cosmid library of Streptomyces clavuligerus was constructed and transferred efficiently by conjugation to Streptomyces lividans, and 12 distinct groups of overlapping cosmid clones that activated the silent actinorhodin biosynthesis gene cluster were identified. This generally applicable high-throughput screening procedure greatly facilitates the identification of antibiotic biosynthesis activators.

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