Performance of the Roche LightCycler Real-time PCR Assay for Diagnosing Extrapulmonary Tuberculosis
Overview
Affiliations
The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management.
Babafemi E, Cherian B, Banting L, Mills G, Ngianga 2nd K Syst Rev. 2017; 6(1):215.
PMID: 29070061 PMC: 5657121. DOI: 10.1186/s13643-017-0608-2.
A differential fluorescent receptor for nucleic acid analysis.
Bengtson H, Kolpashchikov D Chembiochem. 2013; 15(2):228-31.
PMID: 24339354 PMC: 4066444. DOI: 10.1002/cbic.201300657.
Detection of Mycobacterium tuberculosis in blood by use of the Xpert MTB/RIF assay.
Banada P, Koshy R, Alland D J Clin Microbiol. 2013; 51(7):2317-22.
PMID: 23678063 PMC: 3697682. DOI: 10.1128/JCM.00332-13.
Causes of death on antiretroviral therapy: a post-mortem study from South Africa.
Wong E, Omar T, Setlhako G, Osih R, Feldman C, Murdoch D PLoS One. 2012; 7(10):e47542.
PMID: 23094059 PMC: 3472995. DOI: 10.1371/journal.pone.0047542.
Molecular logic gates for DNA analysis: detection of rifampin resistance in M. tuberculosis DNA.
Cornett E, Campbell E, Gulenay G, Peterson E, Bhaskar N, Kolpashchikov D Angew Chem Int Ed Engl. 2012; 51(36):9075-7.
PMID: 22888076 PMC: 3517149. DOI: 10.1002/anie.201203708.