Proteomic Study and Marker Protein Identification of Caenorhabditis Elegans Lipid Droplets

Overview

Journal Mol Cell Proteomics

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2012 Apr 12

PMID 22493183

Citations 67

Authors

Peng Zhang

Huimin Na

Zhenglong Liu

Shuyan Zhang

Peng Xue

Yong Chen

Jing Pu

Gong Peng

Xun Huang

Fuquan Yang

Zhensheng Xie

Tao Xu

Pingyong Xu

Guangshuo Ou

Shaobing O Zhang

Pingsheng Liu

Affiliations

Soon will be listed here.

Abstract

Lipid droplets (LDs) are a neutral lipid storage organelle that is conserved across almost all species. Many metabolic syndromes are directly linked to the over-storage of neutral lipids in LDs. The study of LDs in Caenorhabditis elegans (C. elegans) has been difficult because of the lack of specific LD marker proteins. Here we report the purification and proteomic analysis of C. elegans lipid droplets for the first time. We identified 306 proteins, 63% of these proteins were previously known to be LD-proteins, suggesting a similarity between mammalian and C. elegans LDs. Using morphological and biochemical analyses, we show that short-chain dehydrogenase, DHS-3 is almost exclusively localized on C. elegans LDs, indicating that it can be used as a LD marker protein in C. elegans. These results will facilitate further mechanistic studies of LDs in this powerful genetic system, C. elegans.

Citing Articles

Growth on stiffer substrates impacts animal health and longevity in C. elegans.

Oorloff M, Hruby A, Averbukh M, Alcala A, Dutta N, Minor C PLoS One. 2024; 19(9):e0302673.

PMID: 39264947 PMC: 11392421. DOI: 10.1371/journal.pone.0302673.

Co-immunoprecipitation for identifying protein-protein interaction on lipid droplets.

Fu X, Zhang S, Liu P Biophys Rep. 2024; 10(2):102-110.

PMID: 38774355 PMC: 11103721. DOI: 10.52601/bpr.2024.240007.

Mechanical stress through growth on stiffer substrates impacts animal health and longevity in .

Oorloff M, Hruby A, Averbukh M, Alcala A, Dutta N, Castro Torres T bioRxiv. 2024; .

PMID: 38645203 PMC: 11030433. DOI: 10.1101/2024.04.11.589121.

Proteasome hyperactivation rewires the proteome enhancing stress resistance, proteostasis, lipid metabolism and ERAD in .

Salcedo-Tacuma D, Asad N, Howells G, Anderson R, Smith D bioRxiv. 2024; .

PMID: 38617285 PMC: 11014606. DOI: 10.1101/2024.04.04.588128.

LET-767 determines lipid droplet protein targeting and lipid homeostasis.

Fu L, Zhang J, Wang Y, Wu H, Xu X, Li C J Cell Biol. 2024; 223(6).

PMID: 38551495 PMC: 10982117. DOI: 10.1083/jcb.202311024.

References

Zhang S, Box A, Xu N, Le Men J, Yu J, Guo F . Genetic and dietary regulation of lipid droplet expansion in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2010; 107(10):4640-5. PMC: 2842062. DOI: 10.1073/pnas.0912308107. View

Farese Jr R, Walther T . Lipid droplets finally get a little R-E-S-P-E-C-T. Cell. 2009; 139(5):855-60. PMC: 3097139. DOI: 10.1016/j.cell.2009.11.005. View

Goodman J . The gregarious lipid droplet. J Biol Chem. 2008; 283(42):28005-9. PMC: 2568941. DOI: 10.1074/jbc.R800042200. View

Ding Y, Yang L, Zhang S, Wang Y, Du Y, Pu J . Identification of the major functional proteins of prokaryotic lipid droplets. J Lipid Res. 2011; 53(3):399-411. PMC: 3276463. DOI: 10.1194/jlr.M021899. View

Goodman J . Demonstrated and inferred metabolism associated with cytosolic lipid droplets. J Lipid Res. 2009; 50(11):2148-56. PMC: 2759820. DOI: 10.1194/jlr.R001446. View

Kage-Nakadai E, Kobuna H, Kimura M, Gengyo-Ando K, Inoue T, Arai H . Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans. PLoS One. 2010; 5(1):e8857. PMC: 2810326. DOI: 10.1371/journal.pone.0008857. View

Katavic V, Agrawal G, Hajduch M, Harris S, Thelen J . Protein and lipid composition analysis of oil bodies from two Brassica napus cultivars. Proteomics. 2006; 6(16):4586-98. DOI: 10.1002/pmic.200600020. View

Hellerer T, Axang C, Brackmann C, Hillertz P, Pilon M, Enejder A . Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy. Proc Natl Acad Sci U S A. 2007; 104(37):14658-63. PMC: 1976189. DOI: 10.1073/pnas.0703594104. View

Narbonne P, Roy R . Caenorhabditis elegans dauers need LKB1/AMPK to ration lipid reserves and ensure long-term survival. Nature. 2008; 457(7226):210-4. DOI: 10.1038/nature07536. View

10.

Brooks K, Liang B, Watts J . The influence of bacterial diet on fat storage in C. elegans. PLoS One. 2009; 4(10):e7545. PMC: 2760100. DOI: 10.1371/journal.pone.0007545. View

11.

Szklarczyk D, Franceschini A, Kuhn M, Simonovic M, Roth A, Minguez P . The STRING database in 2011: functional interaction networks of proteins, globally integrated and scored. Nucleic Acids Res. 2010; 39(Database issue):D561-8. PMC: 3013807. DOI: 10.1093/nar/gkq973. View

12.

Yen K, Le T, Bansal A, Narasimhan S, Cheng J, Tissenbaum H . A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods. PLoS One. 2010; 5(9). PMC: 2940797. DOI: 10.1371/journal.pone.0012810. View

13.

Kimmel A, Brasaemle D, McAndrews-Hill M, Sztalryd C, Londos C . Adoption of PERILIPIN as a unifying nomenclature for the mammalian PAT-family of intracellular lipid storage droplet proteins. J Lipid Res. 2009; 51(3):468-71. PMC: 2817576. DOI: 10.1194/jlr.R000034. View

14.

Beller M, Riedel D, Jansch L, Dieterich G, Wehland J, Jackle H . Characterization of the Drosophila lipid droplet subproteome. Mol Cell Proteomics. 2006; 5(6):1082-94. DOI: 10.1074/mcp.M600011-MCP200. View

15.

Brasaemle D, Dolios G, Shapiro L, Wang R . Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes. J Biol Chem. 2004; 279(45):46835-42. DOI: 10.1074/jbc.M409340200. View

16.

Pu J, Ha C, Zhang S, Jung J, Huh W, Liu P . Interactomic study on interaction between lipid droplets and mitochondria. Protein Cell. 2011; 2(6):487-96. PMC: 4875178. DOI: 10.1007/s13238-011-1061-y. View

17.

Zhang S, Trimble R, Guo F, Mak H . Lipid droplets as ubiquitous fat storage organelles in C. elegans. BMC Cell Biol. 2010; 11:96. PMC: 3004847. DOI: 10.1186/1471-2121-11-96. View

18.

Cermelli S, Guo Y, Gross S, Welte M . The lipid-droplet proteome reveals that droplets are a protein-storage depot. Curr Biol. 2006; 16(18):1783-95. DOI: 10.1016/j.cub.2006.07.062. View

19.

Brasaemle D, Wolins N . Packaging of fat: an evolving model of lipid droplet assembly and expansion. J Biol Chem. 2011; 287(4):2273-9. PMC: 3268387. DOI: 10.1074/jbc.R111.309088. View

20.

Schroeder L, Kremer S, Kramer M, Currie E, Kwan E, Watts J . Function of the Caenorhabditis elegans ABC transporter PGP-2 in the biogenesis of a lysosome-related fat storage organelle. Mol Biol Cell. 2007; 18(3):995-1008. PMC: 1805080. DOI: 10.1091/mbc.e06-08-0685. View