Genetic and Dietary Regulation of Lipid Droplet Expansion in Caenorhabditis Elegans

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2010 Feb 24

PMID 20176933

Citations 90

Authors

Shaobing O Zhang

Andrew C Box

Ningyi Xu

Johan Le Men

Jingyi Yu

Fengli Guo

Rhonda Trimble

Ho Yi Mak

Affiliations

Soon will be listed here.

Abstract

Dietary fat accumulates in lipid droplets or endolysosomal compartments that undergo selective expansion under normal or pathophysiological conditions. We find that genetic defects in a peroxisomal beta-oxidation pathway cause size expansion in lipid droplets that are distinct from the lysosome-related organelles in Caenorhabditis elegans. Expansion of lipid droplets is accompanied by an increase in triglycerides (TAG) that are resistant to fasting- or TAG lipase-triggered lipolysis. Nevertheless, in mutant animals, a diet poor in vaccenic acid reduced the TAG level and lipid droplet size. Our results implicate peroxisomal dysfunction in pathologic lipid droplet expansion in animals and illustrate how dietary factors modulate the phenotype of such genetic defects.

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