The Effect of Fresh Bone Marrow Cells on Reconstruction of Mouse Calvarial Defect Combined with Calvarial Osteoprogenitor Cells and Collagen-apatite Scaffold

Overview

Journal J Tissue Eng Regen Med

Specialties Anatomy & Histology
Biotechnology

Date 2012 Apr 5

PMID 22473786

Citations 11

Authors

Xiaohua Yu

Liping Wang

Fei Peng

Xi Jiang

Zengmin Xia

Jianping Huang

David Rowe

Mei Wei

Affiliations

Soon will be listed here.

Abstract

Fresh bone marrow cells have already exhibited its advantages as osteogenic donor cells, but the combination between fresh bone marrow cells and other donor cells utilized for bone healing has not been fully explored. To highlight the impact of fresh bone marrow cells on scaffold-based bone regeneration, single or a combination of calvarial osteoprogenitor cells (OPCs) and bone marrow cells (BMCs) were used as donor cells combined with collagen-apatite scaffold for calvarial defect healing. The host and donor contributions to bone formation were assessed using histological and GFP imaging analysis. Although the amount of new bone formed by different cell sources did not show significant differences, the origin of the bone formation in the defects mainly depended on the types of donor cells employed: when only calvarial OPCs were used as donor cells, a donor-derived bone healing instead of host-derived bone ingrowth was observed; when only fresh BMCs were loaded, the host bone could grow into the defect along the lamellar structure of the scaffolds, but the amount of new bone formed was significantly lower than the defect loaded with calvarial OPCs only. The combination of calvarial OPCs and fresh BMCs had similar amount of new bone formation as the group loaded with calvarial osteoprogenitors alone, but did not induce any host-derived bone formation. These results provide compelling evidence of the importance of fresh BMCs to induce host-implant integration in bone tissue engineering.

Citing Articles

Comparison of osteogenic differentiation potential of induced pluripotent stem cells and buccal fat pad stem cells on 3D-printed HA/β-TCP collagen-coated scaffolds.

Hashemi S, Amirabad L, Farzad-Mohajeri S, Rezai Rad M, Fahimipour F, Ardeshirylajimi A Cell Tissue Res. 2021; 384(2):403-421.

PMID: 33433691 DOI: 10.1007/s00441-020-03374-8.

Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier.

Gohi B, Liu X, Zeng H, Xu S, Ake K, Cao X Cell Biosci. 2020; 10:2.

PMID: 31921407 PMC: 6945441. DOI: 10.1186/s13578-019-0367-y.

Toll-like receptor 4 mediates the regenerative effects of bone grafts for calvarial bone repair.

Wang D, Gilbert J, Shaw M, Shakir S, Losee J, Billiar T Tissue Eng Part A. 2015; 21(7-8):1299-308.

PMID: 25603990 PMC: 4394876. DOI: 10.1089/ten.TEA.2014.0215.

A Biomimetic Collagen-Apatite Scaffold with a Multi-Level Lamellar Structure for Bone Tissue Engineering.

Xia Z, Villa M, Wei M J Mater Chem B. 2014; 2(14):1998-2007.

PMID: 24999428 PMC: 4078891. DOI: 10.1039/C3TB21595D.

Modulation of Host Osseointegration during Bone Regeneration by Controlling Exogenous Stem Cells Differentiation Using a Material Approach.

Yu X, Wang L, Xia Z, Chen L, Jiang X, Rowe D Biomater Sci. 2014; 2(2):242-251.

PMID: 24999385 PMC: 4078879. DOI: 10.1039/C3BM60173K.

References

Augst A, Marolt D, Freed L, Vepari C, Meinel L, Farley M . Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors. J R Soc Interface. 2008; 5(25):929-39. PMC: 2607468. DOI: 10.1098/rsif.2007.1302. View

Wang Y, Liu Y, Buhl K, Rowe D . Comparison of the action of transient and continuous PTH on primary osteoblast cultures expressing differentiation stage-specific GFP. J Bone Miner Res. 2004; 20(1):5-14. DOI: 10.1359/JBMR.041016. View

Boyce B, Yao Z, Xing L . Osteoclasts have multiple roles in bone in addition to bone resorption. Crit Rev Eukaryot Gene Expr. 2009; 19(3):171-80. PMC: 2856465. DOI: 10.1615/critreveukargeneexpr.v19.i3.10. View

Matsuo K . Cross-talk among bone cells. Curr Opin Nephrol Hypertens. 2009; 18(4):292-7. DOI: 10.1097/MNH.0b013e32832b75f1. View

Dumas A, Moreau M, Gherardi R, Basle M, Chappard D . Bone grafts cultured with bone marrow stromal cells for the repair of critical bone defects: an experimental study in mice. J Biomed Mater Res A. 2008; 90(4):1218-29. DOI: 10.1002/jbm.a.32176. View

Yu X, Qu H, Knecht D, Wei M . Incorporation of bovine serum albumin into biomimetic coatings on titanium with high loading efficacy and its release behavior. J Mater Sci Mater Med. 2008; 20(1):287-94. DOI: 10.1007/s10856-008-3571-6. View

Arthur A, Zannettino A, Gronthos S . The therapeutic applications of multipotential mesenchymal/stromal stem cells in skeletal tissue repair. J Cell Physiol. 2008; 218(2):237-45. DOI: 10.1002/jcp.21592. View

Rajan N, Habermehl J, Cote M, Doillon C, Mantovani D . Preparation of ready-to-use, storable and reconstituted type I collagen from rat tail tendon for tissue engineering applications. Nat Protoc. 2007; 1(6):2753-8. DOI: 10.1038/nprot.2006.430. View

Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark S, Lichtler A . Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. J Bone Miner Res. 2002; 17(1):15-25. DOI: 10.1359/jbmr.2002.17.1.15. View

10.

Qu H, Wei M . Improvement of bonding strength between biomimetic apatite coating and substrate. J Biomed Mater Res B Appl Biomater. 2007; 84(2):436-43. DOI: 10.1002/jbm.b.30889. View

11.

Ohgushi H, Goldberg V, Caplan A . Repair of bone defects with marrow cells and porous ceramic. Experiments in rats. Acta Orthop Scand. 1989; 60(3):334-9. DOI: 10.3109/17453678909149289. View

12.

Wang L, Liu Y, Kalajzic Z, Jiang X, Rowe D . Heterogeneity of engrafted bone-lining cells after systemic and local transplantation. Blood. 2005; 106(10):3650-7. PMC: 1895047. DOI: 10.1182/blood-2005-02-0582. View

13.

Heath C . Cells for tissue engineering. Trends Biotechnol. 2000; 18(1):17-9. DOI: 10.1016/s0167-7799(99)01396-7. View

14.

Meinel L, Karageorgiou V, Fajardo R, Snyder B, Shinde-Patil V, Zichner L . Bone tissue engineering using human mesenchymal stem cells: effects of scaffold material and medium flow. Ann Biomed Eng. 2004; 32(1):112-22. DOI: 10.1023/b:abme.0000007796.48329.b4. View

15.

Kalajzic I, Staal A, Yang W, Wu Y, Johnson S, Feyen J . Expression profile of osteoblast lineage at defined stages of differentiation. J Biol Chem. 2005; 280(26):24618-26. DOI: 10.1074/jbc.M413834200. View

16.

Rezwan K, Chen Q, Blaker J, Boccaccini A . Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials. 2006; 27(18):3413-31. DOI: 10.1016/j.biomaterials.2006.01.039. View

17.

Marie P, Debiais F, Cohen-Solal M, De Vernejoul M . New factors controlling bone remodeling. Joint Bone Spine. 2000; 67(3):150-6. View

18.

Kalajzic Z, Li H, Wang L, Jiang X, Lamothe K, Adams D . Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population. Bone. 2008; 43(3):501-10. PMC: 2614133. DOI: 10.1016/j.bone.2008.04.023. View

19.

den Boer F, Wippermann B, Blokhuis T, Patka P, Bakker F, Haarman H . Healing of segmental bone defects with granular porous hydroxyapatite augmented with recombinant human osteogenic protein-1 or autologous bone marrow. J Orthop Res. 2003; 21(3):521-8. DOI: 10.1016/S0736-0266(02)00205-X. View

20.

Langer R, Vacanti J . Tissue engineering. Science. 1993; 260(5110):920-6. DOI: 10.1126/science.8493529. View