A Combinatorial TIR1/AFB-Aux/IAA Co-receptor System for Differential Sensing of Auxin

Overview

Journal Nat Chem Biol

Specialties Biochemistry
Biology
Chemistry

Date 2012 Apr 3

PMID 22466420

Citations 253

Authors

Luz Irina A Calderon Villalobos

Sarah Lee

Cesar de Oliveira

Anthony Ivetac

Wolfgang Brandt

Lynne Armitage

Laura B Sheard

Xu Tan

Geraint Parry

Haibin Mao

Ning Zheng

Richard Napier

Stefan Kepinski

Mark Estelle

Affiliations

Soon will be listed here.

Abstract

The plant hormone auxin regulates virtually every aspect of plant growth and development. Auxin acts by binding the F-box protein transport inhibitor response 1 (TIR1) and promotes the degradation of the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors. Here we show that efficient auxin binding requires assembly of an auxin co-receptor complex consisting of TIR1 and an Aux/IAA protein. Heterologous experiments in yeast and quantitative IAA binding assays using purified proteins showed that different combinations of TIR1 and Aux/IAA proteins form co-receptor complexes with a wide range of auxin-binding affinities. Auxin affinity seems to be largely determined by the Aux/IAA. As there are 6 TIR1/AUXIN SIGNALING F-BOX proteins (AFBs) and 29 Aux/IAA proteins in Arabidopsis thaliana, combinatorial interactions may result in many co-receptors with distinct auxin-sensing properties. We also demonstrate that the AFB5-Aux/IAA co-receptor selectively binds the auxinic herbicide picloram. This co-receptor system broadens the effective concentration range of the hormone and may contribute to the complexity of auxin response.

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