A Novel Mutation in SoIAA20 Confers Cross-resistance to 2,4-Dichlorophenoxyacetic Acid and Other Auxinic Herbicides in Sonchus Oleraceus

Overview

Journal Pest Manag Sci

Specialties Biology
Toxicology

Date 2024 Sep 13

PMID 39268911

Authors

Mahima Krishnan

Tijana Petrovic

Julian G Schwerdt

Alicia B Merriam

James P Hereward

Christopher Preston

Affiliations

Soon will be listed here.

Abstract

Background: 2,4-Dichlorophenoxyacetic acid (2,4-D) and other auxinic herbicides are important for weed control in cropping systems globally. Weeds with resistance to 2,4-D and other auxinic herbicides have evolved, including several populations of Sonchus oleraceus from multiple sites in Australia. We report the underlying mechanism in these populations that gives rise to auxinic herbicide resistance.

Results: We studied a total of three susceptible and eight resistant Sonchus oleraceus populations. All resistant populations had a deletion of three amino acids flanking the degron sequence of an Aux/IAA gene, SoIAA20, which was not found in the three susceptible populations. The eight populations with the resistant allele were also resistant to dicamba, fluroxypyr and clopyralid. The resistant plants also had reduced movement of 2,4-D out of the treated tissues compared to susceptible plants.

Conclusion: The paired deletion flanking the degron region of SoIAA20 likely provides resistance to 2,4-D by restricting the movement of 2,4-D from the treated tissue to the rest of the plant. We hypothesise that this deletion keeps the 2,4-D bound to the target site. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

References

Abel S, Nguyen M, Theologis A . The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. J Mol Biol. 1995; 251(4):533-49. DOI: 10.1006/jmbi.1995.0454. View

Yates A, Allen J, Amode R, Azov A, Barba M, Becerra A . Ensembl Genomes 2022: an expanding genome resource for non-vertebrates. Nucleic Acids Res. 2021; 50(D1):D996-D1003. PMC: 8728113. DOI: 10.1093/nar/gkab1007. View

Hamann T, Mayer U, Jurgens G . The auxin-insensitive bodenlos mutation affects primary root formation and apical-basal patterning in the Arabidopsis embryo. Development. 1999; 126(7):1387-95. DOI: 10.1242/dev.126.7.1387. View

Egan J, Maxwell B, Mortensen D, Ryan M, Smith R . 2,4-Dichlorophenoxyacetic acid (2,4-D)-resistant crops and the potential for evolution of 2,4-D-resistant weeds. Proc Natl Acad Sci U S A. 2011; 108(11):E37. PMC: 3060243. DOI: 10.1073/pnas.1017414108. View

Halliday J, Glickman B . Mechanisms of spontaneous mutation in DNA repair-proficient Escherichia coli. Mutat Res. 1991; 250(1-2):55-71. DOI: 10.1016/0027-5107(91)90162-h. View

Gray W, Kepinski S, Rouse D, Leyser O, Estelle M . Auxin regulates SCF(TIR1)-dependent degradation of AUX/IAA proteins. Nature. 2001; 414(6861):271-6. DOI: 10.1038/35104500. View

Keller O, Kollmar M, Stanke M, Waack S . A novel hybrid gene prediction method employing protein multiple sequence alignments. Bioinformatics. 2011; 27(6):757-63. DOI: 10.1093/bioinformatics/btr010. View

Dreher K, Brown J, Saw R, Callis J . The Arabidopsis Aux/IAA protein family has diversified in degradation and auxin responsiveness. Plant Cell. 2006; 18(3):699-714. PMC: 1383644. DOI: 10.1105/tpc.105.039172. View

Wright T, Shan G, Walsh T, Lira J, Cui C, Song P . Robust crop resistance to broadleaf and grass herbicides provided by aryloxyalkanoate dioxygenase transgenes. Proc Natl Acad Sci U S A. 2010; 107(47):20240-5. PMC: 2996712. DOI: 10.1073/pnas.1013154107. View

10.

LeClere S, Wu C, Westra P, Sammons R . Cross-resistance to dicamba, 2,4-D, and fluroxypyr in is endowed by a mutation in an gene. Proc Natl Acad Sci U S A. 2018; 115(13):E2911-E2920. PMC: 5879655. DOI: 10.1073/pnas.1712372115. View

11.

Paysan-Lafosse T, Blum M, Chuguransky S, Grego T, Pinto B, Salazar G . InterPro in 2022. Nucleic Acids Res. 2022; 51(D1):D418-D427. PMC: 9825450. DOI: 10.1093/nar/gkac993. View

12.

Tan X, Calderon-Villalobos L, Sharon M, Zheng C, Robinson C, Estelle M . Mechanism of auxin perception by the TIR1 ubiquitin ligase. Nature. 2007; 446(7136):640-5. DOI: 10.1038/nature05731. View

13.

Pettinga D, Ou J, Patterson E, Jugulam M, Westra P, Gaines T . Increased chalcone synthase (CHS) expression is associated with dicamba resistance in Kochia scoparia. Pest Manag Sci. 2017; 74(10):2306-2315. DOI: 10.1002/ps.4778. View

14.

Gaines T, Duke S, Morran S, Rigon C, Tranel P, Kupper A . Mechanisms of evolved herbicide resistance. J Biol Chem. 2020; 295(30):10307-10330. PMC: 7383398. DOI: 10.1074/jbc.REV120.013572. View

15.

de Figueiredo M, Kupper A, Malone J, Petrovic T, de Figueiredo A, Campagnola G . An in-frame deletion mutation in the degron tail of auxin coreceptor confers resistance to the herbicide 2,4-D in . Proc Natl Acad Sci U S A. 2022; 119(9). PMC: 8892348. DOI: 10.1073/pnas.2105819119. View

16.

Hamann T, Benkova E, Baurle I, Kientz M, Jurgens G . The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning. Genes Dev. 2002; 16(13):1610-5. PMC: 186366. DOI: 10.1101/gad.229402. View

17.

Calderon Villalobos L, Lee S, de Oliveira C, Ivetac A, Brandt W, Armitage L . A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin. Nat Chem Biol. 2012; 8(5):477-85. PMC: 3331960. DOI: 10.1038/nchembio.926. View

18.

Niemeyer M, Castillo E, Ihling C, Iacobucci C, Wilde V, Hellmuth A . Flexibility of intrinsically disordered degrons in AUX/IAA proteins reinforces auxin co-receptor assemblies. Nat Commun. 2020; 11(1):2277. PMC: 7210949. DOI: 10.1038/s41467-020-16147-2. View

19.

Ghanizadeh H, He L, Griffiths A, Harrington K, Carbone V, Wu H . A novel mutation in IAA16 is associated with dicamba resistance in Chenopodium album. Pest Manag Sci. 2024; 80(7):3675-3683. DOI: 10.1002/ps.8071. View

20.

Dang H, Malone J, Boutsalis P, Krishnan M, Gill G, Preston C . Reduced translocation in 2,4-D-resistant oriental mustard populations (Sisymbrium orientale L.) from Australia. Pest Manag Sci. 2017; 74(6):1524-1532. DOI: 10.1002/ps.4845. View