Retinoic Acid Suppresses Growth of Lesions, Inhibits Peritoneal Cytokine Secretion, and Promotes Macrophage Differentiation in an Immunocompetent Mouse Model of Endometriosis

Overview

Journal Fertil Steril

Specialty Reproductive Medicine

Date 2012 Apr 3

PMID 22464761

Citations 18

Authors

Friedrich Wieser

Juanjuan Wu

Zhaoju Shen

Robert N Taylor

Neil Sidell

Affiliations

Soon will be listed here.

Abstract

Objective: To determine the effects of all-trans-retinoic acid (RA) on establishment and growth of endometrial lesions, peritoneal interleukin-6 (IL-6) and macrophage chemotactic factor-1 (MCP-1) concentrations, and CD38, CD11b, and F4/80 expression on peritoneal macrophages in an immunocompetent mouse model of endometriosis.

Design: Experimental transplantation study using mice.

Setting: Academic medical center.

Animal(s): C57BL/6 recipient mice and syngeneic green fluorescent protein transgenic (GFP+) mice.

Intervention(s): Recipient mice were inoculated with GFP+ minced uterine tissue to induce endometriosis and treated with RA (400 nmol/day) or vehicle for 17 days (3 days before to 14 days after tissue injection).

Main Outcome Measure(s): Total number of GFP+ implants in recipient mice, number of implants showing visible blood vessels, total volume of established lesions per mouse, concentrations of IL-6 and MCP-1 in peritoneal fluid, and expression of CD11b, F4/80, and CD38 on peritoneal macrophages.

Result(s): Retinoic acid treatment for 17 days reduced the number of implants versus controls and decreased the frequency of lesions with vessels. Peritoneal washings in RA-treated animals had lower concentrations of IL-6 and MCP-1 than controls 3 days after endometrial inoculation and lower levels of IL-6 on day 14 after inoculation. Concomitant with these effects on day 14, CD38, CD11b, and F4/80 were higher on macrophages from RA-treated mice versus controls.

Conclusion(s): The development of endometriotic implants is inhibited by RA. This effect may be caused, at least in part, by reduced IL-6 and MCP-1 production and enhanced differentiation of peritoneal macrophages.

Citing Articles

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