Effect of 1,25(OH)2D3 and 24,25(OH)2D3 on Calcium Ion Fluxes in Costochondral Chondrocyte Cultures

Overview

Journal Calcif Tissue Int

Specialty Pathology

Date 1990 Oct 1

PMID 2242495

Citations 5

Authors

G G Langston

L D Swain

Z Schwartz

F Del Toro

R Gomez

B D Boyan

Affiliations

Soon will be listed here.

Abstract

Vitamin D3 metabolites have been shown to affect proliferation, differentiation, and maturation of cartilage cells. Previous studies have shown that growth zone chondrocytes respond primarily to 1,25(OH)2D3 whereas resting zone chondrocytes respond primarily to 24,25(OH)2D3. To examine the role of calcium in the mechanism of hormone action, this study examined the effects of the Ca ionophore A23187, 1,25(OH)2D3, and 24,25(OH)2D3 on Ca influx and efflux in growth zone chondrocytes and resting zone chondrocytes derived from the costochondral junction of 125 g rats. Influx was measured as incorporation of 45Ca. Efflux was measured as release of 45Ca from prelabeled cultures into fresh media. The pattern of 45Ca influx in unstimulated (control) cells over the incubation period was different in the two chondrocyte populations, whereas the pattern of efflux was comparable. A23187 induced a rapid influx of 45Ca in both types of chondrocytes which peaked by 3 minutes and was over by 6 minutes. Influx was greatest in the growth zone chondrocytes. Addition of 10(-8)-10(-9) M 1,25(OH)2D3 to growth zone chondrocyte cultures results in a dose-dependent increase in 45Ca influx after 15 minutes. Efflux was stimulated by these concentrations of hormone throughout the incubation period. Addition of 10(-6)-10(-7) M 24,25(OH)2D3 to resting zone chondrocytes resulted in an inhibition in ion efflux between 1 and 6 minutes, with no effect on influx during this period. Efflux returned to control values between 6 and 15 minutes. 45Ca influx was inhibited by these concentrations of hormone from 15 to 30 minutes.(ABSTRACT TRUNCATED AT 250 WORDS)

Citing Articles

MicroRNA Contents in Matrix Vesicles Produced by Growth Plate Chondrocytes are Cell Maturation Dependent.

Lin Z, McClure M, Zhao J, Ramey A, Asmussen N, Hyzy S Sci Rep. 2018; 8(1):3609.

PMID: 29483516 PMC: 5826934. DOI: 10.1038/s41598-018-21517-4.

Phospholipases of mineralization competent cells and matrix vesicles: roles in physiological and pathological mineralizations.

Mebarek S, Abousalham A, Magne D, Do L, Bandorowicz-Pikula J, Pikula S Int J Mol Sci. 2013; 14(3):5036-129.

PMID: 23455471 PMC: 3634480. DOI: 10.3390/ijms14035036.

Prostaglandins are important in thermoregulation of a reptile (Pogona vitticeps).

Seebacher F, Franklin C Proc Biol Sci. 2003; 270 Suppl 1:S50-3.

PMID: 12952634 PMC: 1698025. DOI: 10.1098/rsbl.2003.0007.

Treatment of resting zone chondrocytes with bone morphogenetic protein-2 induces maturation into a phenotype characteristic of growth zone chondrocytes by downregulating responsiveness to 24,25(OH)2D3 and upregulating responsiveness to 1,25-(OH)2D3.

Schwartz Z, Sylvia V, Liu Y, Dean D, Boyan B Endocrine. 1999; 9(3):273-80.

PMID: 10221593 DOI: 10.1385/ENDO:9:3:273.

The effects of 17 beta-estradiol on chondrocyte differentiation are modulated by vitamin D3 metabolites.

Schwartz Z, Finer Y, Nasatzky E, Soskolne W, Dean D, Boyan B Endocrine. 1998; 7(2):209-18.

PMID: 9549047 DOI: 10.1007/BF02778143.

References

Boyan B, Schwartz Z, Carnes Jr D, Ramirez V . The effects of vitamin D metabolites on the plasma and matrix vesicle membranes of growth and resting cartilage cells in vitro. Endocrinology. 1988; 122(6):2851-60. DOI: 10.1210/endo-122-6-2851. View

Feher J, WASSERMAN R . Intestinal calcium-binding protein and calcium absorption in cortisol-treated chicks: effects of vitamin D3 and 1,25-dihydroxyvitamin D3. Endocrinology. 1979; 104(2):547-51. DOI: 10.1210/endo-104-2-547. View

Nemere I, Leathers V, Norman A . 1,25-Dihydroxyvitamin D3-mediated intestinal calcium transport. Biochemical identification of lysosomes containing calcium and calcium-binding protein (calbindin-D28K). J Biol Chem. 1986; 261(34):16106-14. View

Lieberherr M . Effects of vitamin D3 metabolites on cytosolic free calcium in confluent mouse osteoblasts. J Biol Chem. 1987; 262(27):13168-73. DOI: 10.1515/9783110846713.769. View

Boyan B, Schwartz Z, Swain L, Carnes Jr D, Zislis T . Differential expression of phenotype by resting zone and growth region costochondral chondrocytes in vitro. Bone. 1988; 9(3):185-94. DOI: 10.1016/8756-3282(88)90008-7. View

Kretsinger R . Structure and evolution of calcium-modulated proteins. CRC Crit Rev Biochem. 1980; 8(2):119-74. DOI: 10.3109/10409238009105467. View

Rasmussen H, Matsumoto T, Fontaine O, GOODMAN D . Role of changes in membrane lipid structure in the action of 1,25-dihydroxyvitamin D3. Fed Proc. 1982; 41(1):72-7. View

Tanaka Y, DeLuca H . Bone mineral mobilization activity of 1,25-dihydroxycholecalciferol, a metabolite of vitamin D. Arch Biochem Biophys. 1971; 146(2):574-8. DOI: 10.1016/0003-9861(71)90163-9. View

WASSERMAN R, Fullmer C . Calcium transport proteins, calcium absorption, and vitamin D. Annu Rev Physiol. 1983; 45:375-90. DOI: 10.1146/annurev.ph.45.030183.002111. View

10.

Bikle D, Zolock D, Morrissey R, HERMAN R . Independence of 1,25-dihydroxyvitamin D3-mediated calcium transport from de novo RNA and protein synthesis. J Biol Chem. 1978; 253(2):484-8. View

11.

Hale L, Kemick M, Wuthier R . Effect of vitamin D metabolites on the expression of alkaline phosphatase activity by epiphyseal hypertrophic chondrocytes in primary cell culture. J Bone Miner Res. 1986; 1(6):489-95. DOI: 10.1002/jbmr.5650010602. View

12.

Putkey J, Nemere I, Norman A . Vitamin D status and brush border membrane vesicles: 1,25-dihydroxyvitamin D3 induced destabilization. J Bone Miner Res. 1986; 1(4):305-11. DOI: 10.1002/jbmr.5650010402. View

13.

Fullmer C, Brindak M, Edelstein S, WASSERMAN R . Early and direct effect of 1,25-dihydroxycholecalciferol on calcium uptake by duodena of rachitic chicks. Proc Soc Exp Biol Med. 1984; 177(3):455-8. DOI: 10.3181/00379727-177-41972. View

14.

Brasseur R, Ruysschaert J . Conformation and mode of organization of amphiphilic membrane components: a conformational analysis. Biochem J. 1986; 238(1):1-11. PMC: 1147090. DOI: 10.1042/bj2380001. View

15.

Schiffl H, Binswanger U . Calcium ATPase and intestinal calcium transport in uremic rats. Am J Physiol. 1980; 238(5):G424-8. DOI: 10.1152/ajpgi.1980.238.5.G424. View

16.

Yoshimoto Y, Norman A . Biological activity of vitamin D metabolites and analogs: dose-response study of 45Ca transport in an isolated chick duodenum perfusion system. J Steroid Biochem. 1986; 25(6):905-9. DOI: 10.1016/0022-4731(86)90322-5. View

17.

Norman A, Mircheff A, Adams T, Spielvogel A . Studies on the mechanism of action of calciferol. 3. Vitamin D-mediated increase of intestinal brush order alkaline phosphatase activity. Biochim Biophys Acta. 1970; 215(2):348-59. DOI: 10.1016/0304-4165(70)90034-6. View

18.

Dziak R, Brand J . Calcium transport in isolated bone cells. I. Bone cell isolation procedures. J Cell Physiol. 1974; 84(1):75-83. DOI: 10.1002/jcp.1040840109. View

19.

Wells M . A kinetic study of the phospholipase A 2 (Crotalus adamanteus) catalyzed hydrolysis of 1,2-dibutyryl-sn-glycero-3-phosphorylcholine. Biochemistry. 1972; 11(6):1030-41. DOI: 10.1021/bi00756a013. View

20.

Brighton C, HUNT R . Histochemical localization of calcium in growth plate mitochondria and matrix vesicles. Fed Proc. 1976; 35(2):143-7. View