Characterization of DalS, an ATP-binding Cassette Transporter for D-alanine, and Its Role in Pathogenesis in Salmonella Enterica

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2012 Mar 16

PMID 22418438

Citations 12

Authors

Suzanne E Osborne

Brian R Tuinema

Mac C Y Mok

Pui Sai Lau

Nhat Khai Bui

Ana M Tomljenovic-Berube

Waldemar Vollmer

Kun Zhang

Murray Junop

Brian K Coombes

Affiliations

Soon will be listed here.

Abstract

Expansion into new host niches requires bacterial pathogens to adapt to changes in nutrient availability and to evade an arsenal of host defenses. Horizontal acquisition of Salmonella Pathogenicity Island (SPI)-2 permitted the expansion of Salmonella enterica serovar Typhimurium into the intracellular environment of host cells by allowing it to deliver bacterial effector proteins across the phagosome membrane. This is facilitated by the SsrA-SsrB two-component regulatory system and a type III secretion system encoded within SPI-2. SPI-2 acquisition was followed by evolution of existing regulatory DNA, creating an expanded SsrB regulon involved in intracellular fitness and host infection. Here, we identified an SsrB-regulated operon comprising an ABC transporter in Salmonella. Biochemical and structural studies determined that the periplasmic solute-binding component, STM1633/DalS, transports D-alanine and that DalS is required for intracellular survival of the bacteria and for fitness in an animal host. This work exemplifies the role of nutrient exchange at the host-pathogen interface as a critical determinant of disease outcome.

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