Measurement of the Chemotactic Complement Fragment C5a in Rheumatoid Synovial Fluids by Radioimmunoassay: Role of C5a in the Acute Inflammatory Phase

Overview

Journal Ann Rheum Dis

Specialty Rheumatology

Date 1990 Oct 1

PMID 2241262

Citations 50

Authors

P J Jose

I K Moss

R N Maini

T J Williams

Affiliations

Soon will be listed here.

Abstract

Evidence suggests that complement is activated in rheumatoid joints. A sensitive radioimmunoassay for the activation fragment of C5, C5a, which is a potent chemoattractant for neutrophils, was therefore developed. A mean C5a concentration in 22 rheumatoid joint fluids of about 2.5 x 10(-9) mol/l was found. This concentration of C5a is sufficient to induce two of the characteristic features of the acute inflammatory phases of rheumatoid arthritis: neutrophil accumulation and microvascular plasma protein leakage. In animal models it has been shown that C5a is a potent inducer of inflammatory oedema by a neutrophil dependent mechanism. A striking feature of the acute inflammatory phases of rheumatoid arthritis is the appearance of high numbers of neutrophils in the synovial fluid. It is suggested that C5a might have a role in mediating neutrophil accumulation and, as a consequence, may be important in acute joint swelling and pain.

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