The Visualisation and Speed of Kill of Wound Isolates on a Silver Alginate Dressing

Overview

Journal Int Wound J

Specialties Emergency Medicine
Orthopedics

Date 2012 Mar 13

PMID 22405034

Citations 11

Authors

Samuel J Hooper

Steven L Percival

Katja E Hill

David W Thomas

A J Hayes

David W Williams

Affiliations

Soon will be listed here.

Abstract

In chronic wound management, alginate dressings are used to absorb exudate and reduce the microbial burden. Silver alginate offers the added benefit of an additional antimicrobial pressure on contaminating microorganisms. This present study compares the antimicrobial activity of a RESTORE silver alginate dressing with a silver-free control dressing using a combination of in vitro culture and imaging techniques. The wound pathogens examined included Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, β-haemolytic Streptococcus, and strictly anaerobic bacteria. The antimicrobial efficacy of the dressings was assessed using log(10) reduction and 13-day corrected zone of inhibition (CZOI) time-course assays. Confocal laser scanning microscopy (CLSM) was used to visualise the relative proportions of live/dead microorganisms sequestered into the dressings over 24 hours and estimate the comparative speed of kill. The RESTORE silver alginate dressing showed significantly greater log(10) reductions and CZOIs for all microorganisms compared with the control, indicating the antimicrobial effect of ionic silver. Antimicrobial activity was evident against all test organisms for up to 5 days and, in some cases, up to 12 days following an on-going microbial challenge. Imaging bacteria sequestered in the silver-free dressing showed that each microbial species aggregated in the dressing and remained viable for more than 20 hours. Growth was not observed inside of the dressing, indicating a possible microbiostatic effect of the alginate fibres. In comparison, organisms in the RESTORE silver alginate dressing were seen to lose viability at a considerably greater rate. After 16 hours of contact with the RESTORE silver alginate dressing, >90% of cells of all bacteria and yeast were no longer viable. In conclusion, collectively, the data highlights the rapid speed of kill and antimicrobial suitability of this RESTORE silver alginate dressing on wound isolates and highlights its overwhelming ability to manage a microbial wound bioburden in the management of infected wounds.

Citing Articles

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References

Percival S, Bowler P, Russell D . Bacterial resistance to silver in wound care. J Hosp Infect. 2005; 60(1):1-7. DOI: 10.1016/j.jhin.2004.11.014. View

Wolcott R, Rumbaugh K, James G, Schultz G, Phillips P, Yang Q . Biofilm maturity studies indicate sharp debridement opens a time- dependent therapeutic window. J Wound Care. 2010; 19(8):320-8. DOI: 10.12968/jowc.2010.19.8.77709. View

Schultz G, Sibbald R, Falanga V, Ayello E, Dowsett C, Harding K . Wound bed preparation: a systematic approach to wound management. Wound Repair Regen. 2003; 11 Suppl 1:S1-28. DOI: 10.1046/j.1524-475x.11.s2.1.x. View

Andrews J . The development of the BSAC standardized method of disc diffusion testing. J Antimicrob Chemother. 2001; 48 Suppl 1:29-42. DOI: 10.1093/jac/48.suppl_1.29. View

Beele H, Meuleneire F, Nahuys M, Percival S . A prospective randomised open label study to evaluate the potential of a new silver alginate/carboxymethylcellulose antimicrobial wound dressing to promote wound healing. Int Wound J. 2010; 7(4):262-70. PMC: 7951269. DOI: 10.1111/j.1742-481X.2010.00669.x. View

Miller C, Carville K, Newall N, Kapp S, Lewin G, Karimi L . Assessing bacterial burden in wounds: comparing clinical observation and wound swabs. Int Wound J. 2010; 8(1):45-55. PMC: 7950706. DOI: 10.1111/j.1742-481X.2010.00747.x. View

Thomas J, Slone W, Linton S, Okel T, Corum L, Percival S . In vitro antimicrobial efficacy of a silver alginate dressing on burn wound isolates. J Wound Care. 2011; 20(3):124,126-8. DOI: 10.12968/jowc.2011.20.3.124. View

Kim J, Kwon S, Ostler E . Antimicrobial effect of silver-impregnated cellulose: potential for antimicrobial therapy. J Biol Eng. 2009; 3:20. PMC: 2796638. DOI: 10.1186/1754-1611-3-20. View

Howell-Jones R, Wilson M, Hill K, Howard A, Price P, Thomas D . A review of the microbiology, antibiotic usage and resistance in chronic skin wounds. J Antimicrob Chemother. 2005; 55(2):143-9. DOI: 10.1093/jac/dkh513. View

10.

ROBSON M, Mannari R, Smith P, Payne W . Maintenance of wound bacterial balance. Am J Surg. 1999; 178(5):399-402. DOI: 10.1016/s0002-9610(99)00208-1. View

11.

Sen C, Gordillo G, Roy S, Kirsner R, Lambert L, Hunt T . Human skin wounds: a major and snowballing threat to public health and the economy. Wound Repair Regen. 2009; 17(6):763-71. PMC: 2810192. DOI: 10.1111/j.1524-475X.2009.00543.x. View

12.

Schultz G, Barillo D, Mozingo D, Chin G . Wound bed preparation and a brief history of TIME. Int Wound J. 2006; 1(1):19-32. PMC: 7951422. DOI: 10.1111/j.1742-481x.2004.00008.x. View

13.

James G, Swogger E, Wolcott R, deLancey Pulcini E, Secor P, Sestrich J . Biofilms in chronic wounds. Wound Repair Regen. 2007; 16(1):37-44. DOI: 10.1111/j.1524-475X.2007.00321.x. View

14.

Ratliff C, Getchell-White S, Rodeheaver G . Quantitation of bacteria in clean, nonhealing, chronic wounds. Wounds. 2015; 20(10):279-83. View

15.

Woods E, Cochrane C, Percival S . Prevalence of silver resistance genes in bacteria isolated from human and horse wounds. Vet Microbiol. 2009; 138(3-4):325-9. DOI: 10.1016/j.vetmic.2009.03.023. View

16.

Jones V, Grey J, Harding K . Wound dressings. BMJ. 2006; 332(7544):777-80. PMC: 1420733. DOI: 10.1136/bmj.332.7544.777. View

17.

Fonder M, Lazarus G, Cowan D, Aronson-Cook B, Kohli A, Mamelak A . Treating the chronic wound: A practical approach to the care of nonhealing wounds and wound care dressings. J Am Acad Dermatol. 2008; 58(2):185-206. DOI: 10.1016/j.jaad.2007.08.048. View

18.

Shah M, Mohanraj M . High levels of fusidic acid-resistant Staphylococcus aureus in dermatology patients. Br J Dermatol. 2003; 148(5):1018-20. DOI: 10.1046/j.1365-2133.2003.05291.x. View

19.

Landsdown A, Williams A . Bacterial resistance to silver in wound care and medical devices. J Wound Care. 2007; 16(1):15-9. DOI: 10.12968/jowc.2007.16.1.26983. View

20.

Davies C, Hill K, Newcombe R, Stephens P, Wilson M, Harding K . A prospective study of the microbiology of chronic venous leg ulcers to reevaluate the clinical predictive value of tissue biopsies and swabs. Wound Repair Regen. 2007; 15(1):17-22. DOI: 10.1111/j.1524-475X.2006.00180.x. View