Modulation of Biogenic Amines Content by Poly(propylene Imine) Dendrimers in Rats

Overview

Journal J Physiol Biochem

Specialties Biochemistry
Physiology

Date 2012 Feb 29

PMID 22371014

Authors

Karol Ciepluch

Barbara Ziemba

Anna Janaszewska

Dietmar Appelhans

Barbara Klajnert

Maria Bryszewska

Wieslawa Agnieszka Fogel

Affiliations

Soon will be listed here.

Abstract

Biogenic amines and polyamines participate in all vital organism functions, their levels being important function determinants. Studies were performed to check whether repeated administration of poly(propylene imine) (PPI) dendrimers, synthetic macromolecules with diaminobutane core, and peripheral primary amine groups, may influence the endogenous level of amines, as represented by the two of them: spermidine, a natural derivative of diaminobutane, and histamine. The experiment was carried out on Wistar rats. Fourth generation PPI dendrimer, as well as maltotriose-modified fourth generation PPI dendrimers with (a) cationic open sugar shell and (b) neutral dense sugar shell that possess a higher biocompatibility, was used. Applying the combination of column chromatography on Cellex P and spectrofluorimetric assays of o-phthaldialdehyde, the final amine condensation products were employed to analyze tissue spermidine and histamine outside the central nervous system. Furthermore, radioenzymatic assay was used to measure histamine levels in the brain. The obtained results indicate that in some tissues, the endogenous concentrations of histamine and spermidine may be affected by dendrimers depending on their dose and type of dendrimers.

References

Leveque J, Foucher F, Bansard J, Havouis R, Grall J, Moulinoux J . Polyamine profiles in tumor, normal tissue of the homologous breast, blood, and urine of breast cancer sufferers. Breast Cancer Res Treat. 2000; 60(2):99-105. DOI: 10.1023/a:1006319818530. View

Hakanson R, Ronnberg A . Fluorometric determination of spermidine using o-phthalaldehyde: optimum reaction conditions and tests of identity. Anal Biochem. 1973; 54(2):353-61. DOI: 10.1016/0003-2697(73)90363-1. View

Ziemba B, Janaszewska A, Ciepluch K, Krotewicz M, Fogel W, Appelhans D . In vivo toxicity of poly(propyleneimine) dendrimers. J Biomed Mater Res A. 2011; 99(2):261-8. DOI: 10.1002/jbm.a.33196. View

Cipolla B, Guille F, Moulinoux J, Quemener V, Staerman F, Corbel L . Polyamines and prostatic carcinoma: clinical and therapeutic implications. Eur Urol. 1993; 24(1):124-31. DOI: 10.1159/000474279. View

Burns M, Graminski G, Weeks R, Chen Y, OBrien T . Lipophilic lysine-spermine conjugates are potent polyamine transport inhibitors for use in combination with a polyamine biosynthesis inhibitor. J Med Chem. 2009; 52(7):1983-93. PMC: 2714422. DOI: 10.1021/jm801580w. View

Jiang Y, Tang G, Zhang L, Kong S, Zhu S, Pei Y . PEGylated PAMAM dendrimers as a potential drug delivery carrier: in vitro and in vivo comparative evaluation of covalently conjugated drug and noncovalent drug inclusion complex. J Drug Target. 2010; 18(5):389-403. DOI: 10.3109/10611860903494203. View

Roberts J, Bhalgat M, Zera R . Preliminary biological evaluation of polyamidoamine (PAMAM) Starburst dendrimers. J Biomed Mater Res. 1996; 30(1):53-65. DOI: 10.1002/(SICI)1097-4636(199601)30:1<53::AID-JBM8>3.0.CO;2-Q. View

Shaff R, BEAVEN M . Increased sensitivity of the enzymatic isotopic assay of histamine: measurement of histamine in plasma and serum. Anal Biochem. 1979; 94(2):425-30. DOI: 10.1016/0003-2697(79)90385-3. View

Gerner E, Meyskens Jr F . Polyamines and cancer: old molecules, new understanding. Nat Rev Cancer. 2004; 4(10):781-92. DOI: 10.1038/nrc1454. View

10.

Ogasawara M, Yamauchi K, Satoh Y, Yamaji R, Inui K, Jonker J . Recent advances in molecular pharmacology of the histamine systems: organic cation transporters as a histamine transporter and histamine metabolism. J Pharmacol Sci. 2006; 101(1):24-30. DOI: 10.1254/jphs.fmj06001x6. View

11.

Prieto M, Temprana C, del Rio Zabala N, Marotta C, Del Valle Alonso S . Optimization and in vitro toxicity evaluation of G4 PAMAM dendrimer-risperidone complexes. Eur J Med Chem. 2011; 46(3):845-50. DOI: 10.1016/j.ejmech.2010.12.021. View

12.

Neerman M, Zhang W, Parrish A, Simanek E . In vitro and in vivo evaluation of a melamine dendrimer as a vehicle for drug delivery. Int J Pharm. 2004; 281(1-2):129-32. DOI: 10.1016/j.ijpharm.2004.04.023. View

13.

Berlin G . The dynamics of mast cell secretion mediated by IgE or polyamines. Agents Actions. 1984; 15(5-6):482-7. DOI: 10.1007/BF01966760. View

14.

Freed D . Do dietary lectins cause disease?. BMJ. 1999; 318(7190):1023-4. PMC: 1115436. DOI: 10.1136/bmj.318.7190.1023. View

15.

Silla Santos M . Biogenic amines: their importance in foods. Int J Food Microbiol. 1996; 29(2-3):213-31. DOI: 10.1016/0168-1605(95)00032-1. View

16.

Seiler N, Raul F . Polyamines and apoptosis. J Cell Mol Med. 2005; 9(3):623-42. PMC: 6741638. DOI: 10.1111/j.1582-4934.2005.tb00493.x. View

17.

Jones B, Kearns G . Histamine: new thoughts about a familiar mediator. Clin Pharmacol Ther. 2010; 89(2):189-97. DOI: 10.1038/clpt.2010.256. View

18.

He S . Key role of mast cells and their major secretory products in inflammatory bowel disease. World J Gastroenterol. 2004; 10(3):309-18. PMC: 4724914. DOI: 10.3748/wjg.v10.i3.309. View

19.

Klajnert B, Appelhans D, Komber H, Morgner N, Schwarz S, Richter S . The influence of densely organized maltose shells on the biological properties of poly(propylene imine) dendrimers: new effects dependent on hydrogen bonding. Chemistry. 2008; 14(23):7030-41. DOI: 10.1002/chem.200800342. View

20.

Hakanson R, Ronnberg A, Sjolund K . Fluorometric determination of histamine with OPT: optimum reaction conditions and tests of identity. Anal Biochem. 1972; 47(2):356-70. DOI: 10.1016/0003-2697(72)90129-7. View