Acinetobacter Baumannii Outer Membrane Protein A Modulates the Biogenesis of Outer Membrane Vesicles

Overview

Journal J Microbiol

Specialty Microbiology

Date 2012 Feb 28

PMID 22367951

Citations 73

Authors

Dong Chan Moon

Chul Hee Choi

Jung Hwa Lee

Chi-Won Choi

Hye-Yeon Kim

Jeong Soon Park

Seung Il Kim

Je Chul Lee

Affiliations

Soon will be listed here.

Abstract

Acinetobacter baumannii secretes outer membrane vesicles (OMVs) during both in vitro and in vivo growth, but the biogenesis mechanism by which A. baumannii produces OMVs remains undefined. Outer membrane protein A of A. baumannii (AbOmpA) is a major protein in the outer membrane and the C-terminus of AbOmpA interacts with diaminopimelate of peptidoglycan. This study investigated the role of AbOmpA in the biogenesis of A. baumannii OMVs. Quantitative and qualitative approaches were used to analyze OMV biogenesis in A. baumannii ATCC 19606T and an isogenic ΔAbOmpA mutant. OMV production was significantly increased in the ΔAbOmpA mutant compared to wild-type bacteria as demonstrated by quantitation of proteins and lipopolysaccharides (LPS) packaged in OMVs. LPS profiles prepared from OMVs from wild-type bacteria and the ΔAbOmpA mutant had identical patterns, but proteomic analysis showed different protein constituents in OMVs from wild-type bacteria compared to the ΔAbOmpA mutant. In conclusion, AbOmpA influences OMV biogenesis by controlling OMV production and protein composition.

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