Effect of the Heat Shock Protein HSP27 on Androgen Receptor Expression and Function in Prostate Cancer Cells

Overview

Journal World J Urol

Specialty Urology

Date 2012 Feb 25

PMID 22362414

Citations 23

Authors

Matthias B Stope

Tina Schubert

Doreen Staar

Cindy Ronnau

Andreas Streitborger

Nils Kroeger

Constanze Kubisch

Uwe Zimmermann

Reinhard Walther

Martin Burchardt

Affiliations

Soon will be listed here.

Abstract

Purpose: Heat shock proteins (HSP) are involved in processes of folding, activation, trafficking and transcriptional activity of most steroid receptors including the androgen receptor (AR). Accumulating evidence links rising heat shock protein 27 (HSP27) levels with the development of castration-resistant prostate cancer. In order to study the functional relationship between HSP27 and the AR, we modulated the expression of the small heat shock protein HSP27 in human prostate cancer (PC) cell lines.

Methods: HSP27 protein concentrations in LNCaP and PC-3 cells were modulated by over-expression or silencing of HSP27. The effects of HSP27 on AR protein and mRNA levels were monitored by Western blotting and quantitative RT-PCR.

Results: Treatment for the AR-positive LNCaP with HSP27-specific siRNA resulted in a down-regulation of AR levels. This down-regulation of protein was paralleled by a decrease in AR mRNA. Most interestingly, over-expression of HSP27 in PC-3 cells led to a significant increase in AR mRNA although the cells were unable to produce functional AR protein.

Conclusion: The observation that HSP27 is involved in the regulation of AR mRNA by a yet unknown mechanism highlights the complexity of HSP27-AR signaling network.

Citing Articles

Extract Induces Apoptosis via Generation of Reactive Oxygen Species and Inhibition of Heat Shock Protein 27 and Androgen Receptor in Prostate Cancers.

Kim S, Park J, Lee H, Sim D, Ahn C, Park S Int J Mol Sci. 2024; 25(5).

PMID: 38474045 PMC: 10932161. DOI: 10.3390/ijms25052799.

Cheminformatic Analysis and Machine Learning Modeling to Investigate Androgen Receptor Antagonists to Combat Prostate Cancer.

Yu T, Nantasenamat C, Kachenton S, Anuwongcharoen N, Piacham T ACS Omega. 2023; 8(7):6729-6742.

PMID: 36844574 PMC: 9948163. DOI: 10.1021/acsomega.2c07346.

The Crucial Role of AR-V7 in Enzalutamide-Resistance of Castration-Resistant Prostate Cancer.

Zheng Z, Li J, Liu Y, Shi Z, Xuan Z, Yang K Cancers (Basel). 2022; 14(19).

PMID: 36230800 PMC: 9563243. DOI: 10.3390/cancers14194877.

Upregulation of androgen receptor by heat shock protein 27 and miR1 induces pathogenesis of androgenic alopecia.

Wang M, Wang X Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2022; 47(1):72-78.

PMID: 35545365 PMC: 10930487. DOI: 10.11817/j.issn.1672-7347.2022.210131.

Physiological and Genetically Engineered Expression Modulation Methods Do Not Affect Cellular Levels of the Heat Shock Protein HSP60 in Prostate Cancer Cells.

Erb H, Streitborger A, Mustea A, Stope M In Vivo. 2022; 36(2):596-602.

PMID: 35241511 PMC: 8931909. DOI: 10.21873/invivo.12742.

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