Development and Evaluation of a PCR Assay for Tracking the Emergence and Dissemination of Haitian Variant CtxB in Vibrio Cholerae O1 Strains Isolated from Kolkata, India

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2012 Feb 24

PMID 22357499

Citations 38

Authors

Arindam Naha

G P Pazhani

Mou Ganguly

Santanu Ghosh

T Ramamurthy

Ranjan K Nandy

G Balakrish Nair

Yoshifumi Takeda

Asish K Mukhopadhyay

Affiliations

Soon will be listed here.

Abstract

A PCR-based assay was developed to discriminate the classical, El Tor, and Haitian types of ctxB alleles. Our retrospective study using this newly developed PCR showed that Haitian ctxB first appeared in Kolkata during April 2006, and 93.3% of strains isolated during 2011 carried the new allele. Dendrogram analysis showed a pulsed-field gel electrophoresis (PFGE) pattern of the new variant strains isolated recently that was distinct from the PFGE pattern of the strains carrying classical ctxB that closely matched the 2006 to 2007 variant strains.

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