Mesenchymal Stem Cells and Neural Crest Stem Cells from Adult Bone Marrow: Characterization of Their Surprising Similarities and Differences

Overview

Journal Cell Mol Life Sci

Publisher Springer

Specialty Biology

Date 2012 Feb 22

PMID 22349262

Citations 40

Authors

Sabine Wislet-Gendebien

Emerence Laudet

Virginie Neirinckx

Philippe Alix

Pierre Leprince

Aneta Glejzer

Christophe Poulet

Benoit Hennuy

Lukas Sommer

Olga Shakhova

Bernard Rogister

Affiliations

Soon will be listed here.

Abstract

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases.

Citing Articles

CD9/SOX2-positive cells in the intermediate lobe of the rat pituitary gland exhibit mesenchymal stem cell characteristics.

Shindo A, Azuma M, Fujiwara K, Yoshida S, Horiguchi K Cell Tissue Res. 2025; 399(3):277-290.

PMID: 39808267 DOI: 10.1007/s00441-024-03947-x.

Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties.

Smolinska A, Bzinkowska A, Rybkowska P, Chodkowska M, Sarnowska A Stem Cells Int. 2023; 2023:1842958.

PMID: 37771549 PMC: 10533301. DOI: 10.1155/2023/1842958.

Human Bone Marrow Mesenchymal Stem Cells-Derived Exosomal miRNA-21-5p Inhibits Lidocaine-Induced Apoptosis in SH-SY5Y Neuroblastoma Cells.

Chen C, Zhu F, Liu F, Yao Y, Ma Z, Luo S Iran J Public Health. 2023; 52(4):756-765.

PMID: 37551179 PMC: 10404314. DOI: 10.18502/ijph.v52i4.12446.

Mesenchymal Stromal Cell Therapy in Spinal Cord Injury: Mechanisms and Prospects.

Xie J, Wang X, Li M, Tao Z, Teng W, Saijilafu Front Cell Neurosci. 2022; 16:862673.

PMID: 35722621 PMC: 9204037. DOI: 10.3389/fncel.2022.862673.

Proteasome inhibition-enhanced fracture repair is associated with increased mesenchymal progenitor cells in mice.

Zhang H, Li X, Liu J, Lin X, Pei L, Boyce B PLoS One. 2022; 17(2):e0263839.

PMID: 35213543 PMC: 8880819. DOI: 10.1371/journal.pone.0263839.

References

Dorsky R, Moon R, Raible D . Environmental signals and cell fate specification in premigratory neural crest. Bioessays. 2000; 22(8):708-16. DOI: 10.1002/1521-1878(200008)22:8<708::AID-BIES4>3.0.CO;2-N. View

Wislet-Gendebien S, Hans G, Leprince P, Rigo J, Moonen G, Rogister B . Plasticity of cultured mesenchymal stem cells: switch from nestin-positive to excitable neuron-like phenotype. Stem Cells. 2005; 23(3):392-402. DOI: 10.1634/stemcells.2004-0149. View

Kondo T, Matsuoka A, Shimomura A, Koehler K, Chan R, Miller J . Wnt signaling promotes neuronal differentiation from mesenchymal stem cells through activation of Tlx3. Stem Cells. 2011; 29(5):836-46. PMC: 3666870. DOI: 10.1002/stem.624. View

Sieber-Blum M, Grim M, Hu Y, Szeder V . Pluripotent neural crest stem cells in the adult hair follicle. Dev Dyn. 2004; 231(2):258-69. DOI: 10.1002/dvdy.20129. View

Jiang X, Rowitch D, Soriano P, McMahon A, Sucov H . Fate of the mammalian cardiac neural crest. Development. 2000; 127(8):1607-16. DOI: 10.1242/dev.127.8.1607. View

Alvarez-Dolado M, Pardal R, Garcia-Verdugo J, Fike J, Lee H, Pfeffer K . Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Nature. 2003; 425(6961):968-73. DOI: 10.1038/nature02069. View

Sakai Y, Rawson C, Lindburg K, Barnes D . Serum and transforming growth factor beta regulate glial fibrillary acidic protein in serum-free-derived mouse embryo cells. Proc Natl Acad Sci U S A. 1990; 87(21):8378-82. PMC: 54959. DOI: 10.1073/pnas.87.21.8378. View

Kim S, de Vellis J . Stem cell-based cell therapy in neurological diseases: a review. J Neurosci Res. 2009; 87(10):2183-200. DOI: 10.1002/jnr.22054. View

Kalcheim C, Burstyn-Cohen T . Early stages of neural crest ontogeny: formation and regulation of cell delamination. Int J Dev Biol. 2005; 49(2-3):105-16. DOI: 10.1387/ijdb.041949ck. View

10.

Fujiwara K, Mochida S, Matsui A, Nakayama N, Nagoshi S, Toda G . Fulminant hepatitis and late onset hepatic failure in Japan. Hepatol Res. 2008; 38(7):646-57. DOI: 10.1111/j.1872-034X.2008.00322.x. View

11.

Mundell N, Labosky P . Neural crest stem cell multipotency requires Foxd3 to maintain neural potential and repress mesenchymal fates. Development. 2011; 138(4):641-52. PMC: 3026411. DOI: 10.1242/dev.054718. View

12.

Wislet-Gendebien S, Leprince P, Moonen G, Rogister B . Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells. J Cell Sci. 2003; 116(Pt 16):3295-302. DOI: 10.1242/jcs.00639. View

13.

Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori T . Development of mesenchymal stem cells partially originate from the neural crest. Biochem Biophys Res Commun. 2009; 379(4):1114-9. DOI: 10.1016/j.bbrc.2009.01.031. View

14.

Cragnolini A, Huang Y, Gokina P, Friedman W . Nerve growth factor attenuates proliferation of astrocytes via the p75 neurotrophin receptor. Glia. 2009; 57(13):1386-92. PMC: 2735589. DOI: 10.1002/glia.20857. View

15.

Glejzer A, Laudet E, Leprince P, Hennuy B, Poulet C, Shakhova O . Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow. Cell Mol Life Sci. 2010; 68(12):2101-14. PMC: 11114799. DOI: 10.1007/s00018-010-0558-5. View

16.

John N, Cinelli P, Wegner M, Sommer L . Transforming growth factor β-mediated Sox10 suppression controls mesenchymal progenitor generation in neural crest stem cells. Stem Cells. 2011; 29(4):689-99. DOI: 10.1002/stem.607. View

17.

Williams J, Oh S, Jorgensen M, Steiger N, Darwiche H, Shupe T . The role of the Wnt family of secreted proteins in rat oval "stem" cell-based liver regeneration: Wnt1 drives differentiation. Am J Pathol. 2010; 176(6):2732-42. PMC: 2877835. DOI: 10.2353/ajpath.2010.080486. View

18.

Fu Y, Tvrdik P, Makki N, Paxinos G, Watson C . Precerebellar cell groups in the hindbrain of the mouse defined by retrograde tracing and correlated with cumulative Wnt1-cre genetic labeling. Cerebellum. 2011; 10(3):570-84. DOI: 10.1007/s12311-011-0266-1. View

19.

Nagoshi N, Shibata S, Kubota Y, Nakamura M, Nagai Y, Satoh E . Ontogeny and multipotency of neural crest-derived stem cells in mouse bone marrow, dorsal root ganglia, and whisker pad. Cell Stem Cell. 2008; 2(4):392-403. DOI: 10.1016/j.stem.2008.03.005. View

20.

Kleber M, Lee H, Wurdak H, Buchstaller J, Riccomagno M, Ittner L . Neural crest stem cell maintenance by combinatorial Wnt and BMP signaling. J Cell Biol. 2005; 169(2):309-20. PMC: 2171862. DOI: 10.1083/jcb.200411095. View