Phylogenetic Characterization of a Microsporidium (Nosema Sp. MPr) Isolated from the Pieris Rapae

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2012 Feb 9

PMID 22314780

Citations 4

Authors

Darui Chen

Zhongyuan Shen

Feng Zhu

Rui Guan

Jiange Hou

Jiao Zhang

Xiaofang Xu

Xudong Tang

Li Xu

Affiliations

Soon will be listed here.

Abstract

Cabbage butterfly (Pieris rapae), included in the Lepidoptera genus, Pieris family, is the main pest that damages Cruciferae. In this paper, we reported a microsporidian isolate of Nosema species which was isolated from P. rapae in Zhenjiang City, Jiangsu Province, China. The mature spore of this microsporidium is long oval in shape and 3.8 ± 0.3 × 2.0 ± 0.2 μm in size. Research results showed that the novel microsporidium cannot infect the BmN cell in vitro and silkworm larvae. The organization of rRNA gene was 5'-SSU rRNA-ITS-LSU rRNA-3'. Phylogenetic trees based on SSU rRNA and LSU rRNA gene sequences were constructed by MEGA 4.0 software. The topology showed that this microsporidium was on the same second branch of Nosema clade, and had close relationships to other Nosema species. Consequently, this microsporidium was confirmed to be a member of Nosema genus, and named as Nosema sp. MPr.

Citing Articles

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The Genome of Nosema sp. Isolate YNPr: A Comparative Analysis of Genome Evolution within the Nosema/Vairimorpha Clade.

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Determination of Wolbachia diversity in butterflies from Western Ghats, India, by a multigene approach.

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References

Xu X, Shen Z, Zhu F, Tao H, Tang X, Xu L . Phylogenetic characterization of a microsporidium (Endoreticulatus sp. Zhenjiang) isolated from the silkworm, Bombyx mori. Parasitol Res. 2011; 110(2):815-9. DOI: 10.1007/s00436-011-2560-8. View

Rao S, Muthulakshmi M, Kanginakudru S, Nagaraju J . Phylogenetic relationships of three new microsporidian isolates from the silkworm, Bombyx mori. J Invertebr Pathol. 2004; 86(3):87-95. DOI: 10.1016/j.jip.2004.05.004. View

Gatehouse H, Malone L . The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size. J Invertebr Pathol. 1998; 71(2):97-105. DOI: 10.1006/jipa.1997.4737. View

Peyretaillade E, Biderre C, Peyret P, Duffieux F, Metenier G, Gouy M . Microsporidian Encephalitozoon cuniculi, a unicellular eukaryote with an unusual chromosomal dispersion of ribosomal genes and a LSU rRNA reduced to the universal core. Nucleic Acids Res. 1998; 26(15):3513-20. PMC: 147740. DOI: 10.1093/nar/26.15.3513. View

Gresoviac S, Khattra J, Nadler S, Kent M, Devlin R, Vivares C . Comparison of small subunit ribosomal RNA gene and internal transcribed spacer sequences among isolates of the intranuclear microsporidian Nucleospora salmonis. J Eukaryot Microbiol. 2001; 47(4):379-87. DOI: 10.1111/j.1550-7408.2000.tb00064.x. View

Sakova K, Sak B, Ditrich O, Kvac M . Humoral response of chicken infected with the microsporidium Encephalitozoon hellem. Parasitol Res. 2006; 98(5):488-92. DOI: 10.1007/s00436-005-0089-4. View

Franzen C, Muller A, Hartmann P, Hegener P, Schrappe M, Diehl V . Polymerase chain reaction for diagnosis and species differentiation of microsporidia. Folia Parasitol (Praha). 1998; 45(2):140-8. View

Tsai S, Kou G, Lo C, Wang C . Complete sequence and structure of ribosomal RNA gene of Heterosporis anguillarum. Dis Aquat Organ. 2002; 49(3):199-206. DOI: 10.3354/dao049199. View

Tsai S, Lo C, Soichi Y, Wang C . The characterization of microsporidian isolates (Nosematidae: Nosema) from five important lepidopteran pests in Taiwan. J Invertebr Pathol. 2003; 83(1):51-9. DOI: 10.1016/s0022-2011(03)00035-1. View

10.

Muller A, Trammer T, Chioralia G, Seitz H, Diehl V, Franzen C . Ribosomal RNA of Nosema algerae and phylogenetic relationship to other microsporidia. Parasitol Res. 2000; 86(1):18-23. DOI: 10.1007/pl00008501. View

11.

Adl S, Simpson A, Farmer M, Andersen R, Anderson O, Barta J . The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J Eukaryot Microbiol. 2005; 52(5):399-451. DOI: 10.1111/j.1550-7408.2005.00053.x. View

12.

Huang W, Bocquet M, Lee K, Sung I, Jiang J, Chen Y . The comparison of rDNA spacer regions of Nosema ceranae isolates from different hosts and locations. J Invertebr Pathol. 2007; 97(1):9-13. DOI: 10.1016/j.jip.2007.07.001. View

13.

Hibbett D, Binder M, Bischoff J, Blackwell M, Cannon P, Eriksson O . A higher-level phylogenetic classification of the Fungi. Mycol Res. 2007; 111(Pt 5):509-47. DOI: 10.1016/j.mycres.2007.03.004. View

14.

Zhu X, Wittner M, Tanowitz H, Cali A, Weiss L . Ribosomal RNA sequences of Enterocytozoon bieneusi, Septata intestinalis and Ameson michaelis: phylogenetic construction and structural correspondence. J Eukaryot Microbiol. 1994; 41(3):204-9. DOI: 10.1111/j.1550-7408.1994.tb01498.x. View

15.

Nilsen F, Endresen C, Hordvik I . Molecular phylogeny of microsporidians with particular reference to species that infect the muscles of fish. J Eukaryot Microbiol. 1998; 45(5):535-43. DOI: 10.1111/j.1550-7408.1998.tb05113.x. View

16.

Ku C, Wang C, Tsai Y, Tzeng C, Wang C . Phylogenetic analysis of two putative Nosema isolates from Cruciferous Lepidopteran pests in Taiwan. J Invertebr Pathol. 2007; 95(1):71-6. DOI: 10.1016/j.jip.2006.11.008. View

17.

Keeling P, Luker M, Palmer J . Evidence from beta-tubulin phylogeny that microsporidia evolved from within the fungi. Mol Biol Evol. 2000; 17(1):23-31. DOI: 10.1093/oxfordjournals.molbev.a026235. View

18.

Johny S, Kanginakudru S, Muralirangan M, Nagaraju J . Morphological and molecular characterization of a new microsporidian (Protozoa: Microsporidia) isolated from Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). Parasitology. 2006; 132(Pt 6):803-14. DOI: 10.1017/S0031182006009863. View

19.

Desser S, Hong H, Yang Y . Ultrastructure of the development of a species of Encephalitozoon cultured from the eye of an AIDS patient. Parasitol Res. 1992; 78(8):677-83. DOI: 10.1007/BF00931520. View

20.

Zhu F, Shen Z, Xu X, Tao H, Dong S, Tang X . Phylogenetic analysis of complete rRNA gene sequence of Nosema philosamiae isolated from the lepidopteran Philosamia cynthia ricini. J Eukaryot Microbiol. 2010; 57(3):294-6. DOI: 10.1111/j.1550-7408.2010.00475.x. View