Demonstration of Osteocytic Perilacunar/canalicular Remodeling in Mice During Lactation

Overview

Journal J Bone Miner Res

Publisher Oxford University Press

Date 2012 Feb 7

PMID 22308018

Citations 237

Authors

Hai Qing

Laleh Ardeshirpour

Paola Divieti Pajevic

Vladimir Dusevich

Katharina Jahn

Shigeaki Kato

John Wysolmerski

Lynda F Bonewald

Affiliations

Soon will be listed here.

Abstract

Osteoclasts are thought to be solely responsible for the removal of bone matrix. However, we show here that osteocytes can also remove bone matrix by reversibly remodeling their perilacunar/canalicular matrix during the reproductive cycle. In contrast, no osteocytic remodeling was observed with experimental unloading despite similar degrees of bone loss. Gene array analysis of osteocytes from lactating animals revealed an elevation of genes known to be utilized by osteoclasts to remove bone, including tartrate-resistant acid phosphatase (TRAP) and cathepsin K, that returned to virgin levels upon weaning. Infusion of parathyroid hormone-related peptide (PTHrP), known to be elevated during lactation, induced TRAP activity and cathepsin K expression in osteocytes concurrent with osteocytic remodeling. Conversely, animals lacking the parathyroid hormone type 1 receptor (PTHR1) in osteocytes failed to express TRAP or cathepsin K or to remodel their osteocyte perilacunar matrix during lactation. These studies show that osteocytes remove mineralized matrix through molecular mechanisms similar to those utilized by osteoclasts.

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