Rhizobium Meliloti Chromosomal Loci Required for Suppression of Exopolysaccharide Mutations by Lipopolysaccharide

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 Nov 1

PMID 2228976

Citations 7

Authors

M N Williams

R I Hollingsworth

P M Brzoska

E R Signer

Affiliations

Soon will be listed here.

Abstract

Mutants of alfalfa symbiont Rhizobium meliloti SU47 that fail to make extracellular polysaccharide (exo mutants) induce the formation of nodules that are devoid of bacteria and consequently do not fix nitrogen. This Fix- phenotype can be suppressed by an R. meliloti Rm41 gene that affects lipopolysaccharide structure. Here we describe mutations preventing suppression that map at two new chromosomal loci, lpsY and lpsX, present in both strains. Two other lps mutations isolated previously from SU47 also prevented suppression.

Citing Articles

A Bifunctional UDP-Sugar 4-Epimerase Supports Biosynthesis of Multiple Cell Surface Polysaccharides in Sinorhizobium meliloti.

Schaper S, Wendt H, Bamberger J, Sieber V, Schmid J, Becker A J Bacteriol. 2019; 201(10).

PMID: 30833352 PMC: 6482925. DOI: 10.1128/JB.00801-18.

Different phenotypic classes of Sinorhizobium meliloti mutants defective in synthesis of K antigen.

Campbell G, Reuhs B, Walker G J Bacteriol. 1998; 180(20):5432-6.

PMID: 9765576 PMC: 107593. DOI: 10.1128/JB.180.20.5432-5436.1998.

The "missing" typical Rhizobium leguminosarum O antigen is attached to a fatty acylated glycerol in R. leguminosarum bv. trifolii 4S, a strain that also lacks the usual tetrasaccharide "core" component.

Cedergren R, Wang Y, Hollingsworth R J Bacteriol. 1996; 178(18):5529-32.

PMID: 8808946 PMC: 178379. DOI: 10.1128/jb.178.18.5529-5532.1996.

Rhizobium fredii and Rhizobium meliloti produce 3-deoxy-D-manno-2-octulosonic acid-containing polysaccharides that are structurally analogous to group II K antigens (capsular polysaccharides) found in Escherichia coli.

Reuhs B, Carlson R, Kim J J Bacteriol. 1993; 175(11):3570-80.

PMID: 8501061 PMC: 204758. DOI: 10.1128/jb.175.11.3570-3580.1993.

Suppression of the Fix- phenotype of Rhizobium meliloti exoB mutants by lpsZ is correlated to a modified expression of the K polysaccharide.

Reuhs B, Williams M, Kim J, Carlson R, Cote F J Bacteriol. 1995; 177(15):4289-96.

PMID: 7635814 PMC: 177175. DOI: 10.1128/jb.177.15.4289-4296.1995.

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