The Symbiotic Defect of Rhizobium Meliloti Exopolysaccharide Mutants is Suppressed by LpsZ+, a Gene Involved in Lipopolysaccharide Biosynthesis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 May 1

PMID 2158975

Citations 26

Authors

M N Williams

R I Hollingsworth

S Klein

E R Signer

Affiliations

Soon will be listed here.

Abstract

exo mutants of Rhizobium meliloti SU47, which fail to secrete acidic extracellular polysaccharide (EPS), induce Fix- nodules on alfalfa. However, mutants of R. meliloti Rm41 carrying the same exo lesions induce normal Fix+ nodules. We show that such induction is due to a gene from strain Rm41, which we call lpsZ+, that is missing in strain SU47. lpsZ+ does not restore EPS production but instead alters the composition and structure of lipopolysaccharide. In both SU47 and Rm41, either lpsZ+ or exo+ is sufficient for normal nodulation. This suggests that in R. meliloti EPS and lipopolysaccharide can perform the same function in nodule development.

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