Multiplexed Nanoflares: MRNA Detection in Live Cells

Overview

Journal Anal Chem

Specialty Chemistry

Date 2012 Feb 1

PMID 22288418

Citations 65

Authors

Andrew E Prigodich

Pratik S Randeria

William E Briley

Nathaniel J Kim

Weston L Daniel

David A Giljohann

Chad A Mirkin

Affiliations

Soon will be listed here.

Abstract

We report the development of the multiplexed nanoflare, a nanoparticle agent that is capable of simultaneously detecting two distinct mRNA targets inside a living cell. These probes are spherical nucleic acid (SNA) gold nanoparticle (Au NP) conjugates consisting of densely packed and highly oriented oligonucleotide sequences, many of which are hybridized to a reporter with a distinct fluorophore label and each complementary to its corresponding mRNA target. When multiplexed nanoflares are exposed to their targets, they provide a sequence specific signal in both extra- and intracellular environments. Importantly, one of the targets can be used as an internal control, improving detection by accounting for cell-to-cell variations in nanoparticle uptake and background. Compared to single-component nanoflares, these structures allow one to determine more precisely relative mRNA levels in individual cells, improving cell sorting and quantification.

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