Polyvalent DNA Nanoparticle Conjugates Stabilize Nucleic Acids

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2008 Dec 23

PMID 19099465

Citations 176

Authors

Dwight S Seferos

Andrew E Prigodich

David A Giljohann

Pinal C Patel

Chad A Mirkin

Affiliations

Soon will be listed here.

Abstract

Polyvalent oligonucleotide gold nanoparticle conjugates have unique fundamental properties including distance-dependent plasmon coupling, enhanced binding affinity, and the ability to enter cells and resist enzymatic degradation. Stability in the presence of enzymes is a key consideration for therapeutic uses; however the manner and mechanism by which such nanoparticles are able to resist enzymatic degradation is unknown. Here, we quantify the enhanced stability of polyvalent gold oligonucleotide nanoparticle conjugates with respect to enzyme-catalyzed hydrolysis of DNA and present evidence that the negatively charged surfaces of the nanoparticles and resultant high local salt concentrations are responsible for enhanced stability.

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References

SHACK J . The influence of sodium and magnesium ions on the action of deoxyribonuclease II. J Biol Chem. 1959; 234:3003-6. View

Rosi N, Giljohann D, Thaxton C, Lytton-Jean A, Han M, Mirkin C . Oligonucleotide-modified gold nanoparticles for intracellular gene regulation. Science. 2006; 312(5776):1027-30. DOI: 10.1126/science.1125559. View

Xiao Y, Patolsky F, Katz E, Hainfeld J, Willner I . "Plugging into Enzymes": nanowiring of redox enzymes by a gold nanoparticle. Science. 2003; 299(5614):1877-81. DOI: 10.1126/science.1080664. View

Pan C, Lazarus R . Engineering hyperactive variants of human deoxyribonuclease I by altering its functional mechanism. Biochemistry. 1997; 36(22):6624-32. DOI: 10.1021/bi962960x. View

Seferos D, Giljohann D, Rosi N, Mirkin C . Locked nucleic acid-nanoparticle conjugates. Chembiochem. 2007; 8(11):1230-2. DOI: 10.1002/cbic.200700262. View

Thompson D, Enright A, Faulds K, Smith W, Graham D . Ultrasensitive DNA detection using oligonucleotide-silver nanoparticle conjugates. Anal Chem. 2008; 80(8):2805-10. DOI: 10.1021/ac702403w. View

Liu G, Yin Y, Kunchakarra S, Mukherjee B, Gerion D, Jett S . A nanoplasmonic molecular ruler for measuring nuclease activity and DNA footprinting. Nat Nanotechnol. 2008; 1(1):47-52. DOI: 10.1038/nnano.2006.51. View

Demers L, Mirkin C, Mucic R, Reynolds 3rd R, LETSINGER R, Elghanian R . A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. Anal Chem. 2000; 72(22):5535-41. DOI: 10.1021/ac0006627. View

Han G, Martin C, Rotello V . Stability of gold nanoparticle-bound DNA toward biological, physical, and chemical agents. Chem Biol Drug Des. 2006; 67(1):78-82. DOI: 10.1111/j.1747-0285.2005.00324.x. View

10.

Liu J, Lu Y . A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles. J Am Chem Soc. 2003; 125(22):6642-3. DOI: 10.1021/ja034775u. View

11.

Hurst S, Lytton-Jean A, Mirkin C . Maximizing DNA loading on a range of gold nanoparticle sizes. Anal Chem. 2006; 78(24):8313-8. PMC: 2525614. DOI: 10.1021/ac0613582. View

12.

He X, Wang K, Tan W, Liu B, Lin X, He C . Bioconjugated nanoparticles for DNA protection from cleavage. J Am Chem Soc. 2003; 125(24):7168-9. DOI: 10.1021/ja034450d. View

13.

Zaupa G, Scrimin P, Prins L . Origin of the dendritic effect in multivalent enzyme-like catalysts. J Am Chem Soc. 2008; 130(17):5699-709. DOI: 10.1021/ja7113213. View

14.

Elghanian R, Storhoff J, Mucic R, LETSINGER R, Mirkin C . Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science. 1997; 277(5329):1078-81. DOI: 10.1126/science.277.5329.1078. View

15.

You C, Agasti S, De M, Knapp M, Rotello V . Modulation of the catalytic behavior of alpha-chymotrypsin at monolayer-protected nanoparticle surfaces. J Am Chem Soc. 2006; 128(45):14612-8. DOI: 10.1021/ja064433z. View

16.

Tkachenko A, Xie H, Coleman D, Glomm W, Ryan J, Anderson M . Multifunctional gold nanoparticle-peptide complexes for nuclear targeting. J Am Chem Soc. 2003; 125(16):4700-1. DOI: 10.1021/ja0296935. View

17.

Medley C, Smith J, Tang Z, Wu Y, Bamrungsap S, Tan W . Gold nanoparticle-based colorimetric assay for the direct detection of cancerous cells. Anal Chem. 2008; 80(4):1067-72. DOI: 10.1021/ac702037y. View

18.

Lee J, Seferos D, Giljohann D, Mirkin C . Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates. J Am Chem Soc. 2008; 130(16):5430-1. PMC: 8281389. DOI: 10.1021/ja800797h. View

19.

Jin R, Wu G, Li Z, Mirkin C, Schatz G . What controls the melting properties of DNA-linked gold nanoparticle assemblies?. J Am Chem Soc. 2003; 125(6):1643-54. DOI: 10.1021/ja021096v. View

20.

Mirkin C, LETSINGER R, Mucic R, Storhoff J . A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature. 1996; 382(6592):607-9. DOI: 10.1038/382607a0. View