Mycoplasmas Induce Transcription and Production of Tumor Necrosis Factor in a Monocytic Cell Line, THP-1, by a Protein Kinase C-independent Pathway

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1990 Nov 1

PMID 2228228

Citations 15

Authors

K Sugama

K Kuwano

M Furukawa

Y Himeno

T Satoh

S Arai

Affiliations

Soon will be listed here.

Abstract

We demonstrated that mycoplasmas (MP), previously shown to augment the antitumor activity of murine peritoneal macrophages, also induce cytotoxic activity in a human monocytic cell line, THP-1. THP-1 cells were induced to produce cytotoxic activity by MP in a time- and dose-dependent manner. By using neutralization by antibody against tumor necrosis factor (TNF), the cytotoxic activity was shown to be due to TNF released from the MP-stimulated cells. Studies with inhibitors of second-messenger pathways and Northern RNA blot analysis indicated that a Ca2(+)-dependent, but not protein kinase C-dependent, biochemical pathway is involved in MP-induced TNF production by THP-1 cells and that MP induce TNF production in the cells at the level of transcription. MP, unlike other bacteria, lack cell walls and lipopolysaccharide. The possible involvement of a TNF production mechanism distinct from that triggered by lipopolysaccharide is discussed.

Citing Articles

Mycoplasma fermentans inhibits the activity of cellular DNA topoisomerase I by activation of PARP1 and alters the efficacy of its anti-cancer inhibitor.

Afriat R, Horowitz S, Priel E PLoS One. 2013; 8(8):e72377.

PMID: 24013388 PMC: 3754970. DOI: 10.1371/journal.pone.0072377.

Lipid-associated membrane proteins of Mycoplasma fermentans and M. penetrans activate human immunodeficiency virus long-terminal repeats through Toll-like receptors.

Shimizu T, Kida Y, Kuwano K Immunology. 2004; 113(1):121-9.

PMID: 15312143 PMC: 1782549. DOI: 10.1111/j.1365-2567.2004.01937.x.

Opposing roles of activator protein-1 and CCAAT/enhancer binding protein beta in the regulation of inducible granulysin gene expression in a human monocytic cell line, THP-1.

Kida Y, Shimizu T, Kuwano K Immunology. 2002; 107(4):507-16.

PMID: 12460196 PMC: 1782811. DOI: 10.1046/j.1365-2567.2002.01524.x.

Acholeplasma laidlawii up-regulates granulysin gene expression via transcription factor activator protein-1 in a human monocytic cell line, THP-1.

Kida Y, Kuwano K, Zhang Y, Arai S Immunology. 2001; 104(3):324-32.

PMID: 11722647 PMC: 1783303. DOI: 10.1046/j.1365-2567.2001.01310.x.

Close association between pulmonary disease manifestation in Mycoplasma pneumoniae infection and enhanced local production of interleukin-18 in the lung, independent of gamma interferon.

Narita M, Tanaka H, Abe S, Yamada S, Kubota M, Togashi T Clin Diagn Lab Immunol. 2000; 7(6):909-14.

PMID: 11063497 PMC: 95984. DOI: 10.1128/CDLI.7.6.909-914.2000.

References

Hidaka H, Inagaki M, Kawamoto S, Sasaki Y . Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984; 23(21):5036-41. DOI: 10.1021/bi00316a032. View

Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T . Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 1982; 42(4):1530-6. View

Armstrong C, Klostergaard J, GRANGER G . Isolation and initial characterization of tumoricidal monokine(s) from the human monocytic leukemia cell line THP-1. J Natl Cancer Inst. 1985; 74(1):1-9. View

Beutler B, Cerami A . Cachectin and tumour necrosis factor as two sides of the same biological coin. Nature. 1986; 320(6063):584-8. DOI: 10.1038/320584a0. View

Nakamura T, Hemmi H, Aso H, Ishida N . Variants of a human monocytic leukemia cell line (THP-1): induction of differentiation by retinoic acid, interferon-gamma, and T-lymphocyte-derived differentiation-inducing activity. J Natl Cancer Inst. 1986; 77(1):21-7. View

Philip R, Epstein L . Tumour necrosis factor as immunomodulator and mediator of monocyte cytotoxicity induced by itself, gamma-interferon and interleukin-1. Nature. 1986; 323(6083):86-9. DOI: 10.1038/323086a0. View

Introna M, Hamilton T, KAUFMAN R, Adams D, Bast Jr R . Treatment of murine peritoneal macrophages with bacterial lipopolysaccharide alters expression of c-fos and c-myc oncogenes. J Immunol. 1986; 137(8):2711-5. View

Takeda K, Iwamoto S, Sugimoto H, Takuma T, Kawatani N, Noda M . Identity of differentiation inducing factor and tumour necrosis factor. Nature. 1986; 323(6086):338-40. DOI: 10.1038/323338a0. View

Isakov N, Mally M, Scholz W, Altman A . T-lymphocyte activation: the role of protein kinase C and the bifurcating inositol phospholipid signal transduction pathway. Immunol Rev. 1987; 95:89-111. DOI: 10.1111/j.1600-065x.1987.tb00501.x. View

10.

Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9. DOI: 10.1006/abio.1987.9999. View

11.

Larsson L, Ivhed I, Gidlund M, Pettersson U, Vennstrom B, Nilsson K . Phorbol ester-induced terminal differentiation is inhibited in human U-937 monoblastic cells expressing a v-myc oncogene. Proc Natl Acad Sci U S A. 1988; 85(8):2638-42. PMC: 280053. DOI: 10.1073/pnas.85.8.2638. View

12.

Burchett S, WEAVER W, Westall J, Larsen A, KRONHEIM S, Wilson C . Regulation of tumor necrosis factor/cachectin and IL-1 secretion in human mononuclear phagocytes. J Immunol. 1988; 140(10):3473-81. View

13.

TANNENBAUM C, Koerner T, Jansen M, Hamilton T . Characterization of lipopolysaccharide-induced macrophage gene expression. J Immunol. 1988; 140(10):3640-5. View

14.

Kiener P, Marek F, Rodgers G, Lin P, Warr G, Desiderio J . Induction of tumor necrosis factor, IFN-gamma, and acute lethality in mice by toxic and non-toxic forms of lipid A. J Immunol. 1988; 141(3):870-4. View

15.

Himeno Y, Fukuda Y, Hatanaka M, Imura H . Expression of oncogenes in human liver disease. Liver. 1988; 8(4):208-12. DOI: 10.1111/j.1600-0676.1988.tb00994.x. View

16.

Hampton R, Golenbock D, Raetz C . Lipid A binding sites in membranes of macrophage tumor cells. J Biol Chem. 1988; 263(29):14802-7. View

17.

Holian A, Jordan M, Nguyen H, Devenyi Z . Inhibition of macrophage activation by isoquinolinesulfonamides, phenothiazines, and a napthalenesulfonamide. J Cell Physiol. 1988; 137(1):45-54. DOI: 10.1002/jcp.1041370106. View

18.

Renz H, Gong J, Schmidt A, Nain M, Gemsa D . Release of tumor necrosis factor-alpha from macrophages. Enhancement and suppression are dose-dependently regulated by prostaglandin E2 and cyclic nucleotides. J Immunol. 1988; 141(7):2388-93. View

19.

Kovacs E, Radzioch D, Young H, Varesio L . Differential inhibition of IL-1 and TNF-alpha mRNA expression by agents which block second messenger pathways in murine macrophages. J Immunol. 1988; 141(9):3101-5. View

20.

Gallily R, Sher T, Loewenstein J . Tumor necrosis factor as a mediator of Mycoplasma orale-induced tumor cell lysis by macrophages. Cell Immunol. 1989; 121(1):146-53. DOI: 10.1016/0008-8749(89)90012-9. View