Identification of Hydrophobic Tags for the Degradation of Stabilized Proteins

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2012 Jan 25

PMID 22271667

Citations 36

Authors

Hyun Seop Tae

Thomas B Sundberg

Taavi K Neklesa

Devin J Noblin

Jeffrey L Gustafson

Anke G Roth

Kanak Raina

Craig M Crews

Affiliations

Soon will be listed here.

Abstract

New HyTs are a knockout: we previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.

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