Localization and Imaging of Gangliosides in Mouse Brain Tissue Sections by Laserspray Ionization Inlet

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2012 Jan 21

PMID 22262808

Citations 13

Authors

Alicia L Richards

Christopher B Lietz

James Wager-Miller

Ken Mackie

Sarah Trimpin

Affiliations

Soon will be listed here.

Abstract

A new ionization method for the analysis of fragile gangliosides without undesired fragmentation or salt adduction is presented. In laserspray ionization inlet (LSII), the matrix/analyte sample is ablated at atmospheric pressure, and ionization takes place in the ion transfer capillary of the mass spectrometer inlet by a process that is independent of a laser wavelength or voltage. The softness of LSII allows the identification of gangliosides up to GQ1 with negligible sialic acid loss. This is of importance to the field of MS imaging, as undesired fragmentation has made it difficult to accurately map the spatial distribution of fragile ganglioside lipids in tissue. Proof-of-principle structural characterization of endogenous gangliosides using MS(n) fragmentation of multiply charged negative ions on a LTQ Velos and subsequent imaging of the GD1 ganglioside is demonstrated. This is the first report of multiply charged negative ions using inlet ionization. We find that GD1 is detected at higher levels in the mouse cortex and hippocampus compared with the thalamus. In LSII with the laser aligned in transmission geometry relative to the inlet, images were obtained in approximately 60 min using an inexpensive nitrogen laser.

Citing Articles

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References

Inutan E, Trimpin S . Laserspray ionization-ion mobility spectrometry-mass spectrometry: baseline separation of isomeric amyloids without the use of solvents desorbed and Ionized directly from a surface. J Proteome Res. 2010; 9(11):6077-81. DOI: 10.1021/pr1005923. View

Jackson S, Colsch B, Egan T, Lewis E, Schultz J, Woods A . Gangliosides' analysis by MALDI-ion mobility MS. Analyst. 2010; 136(3):463-6. PMC: 7543062. DOI: 10.1039/c0an00732c. View

Fitzgerald M, Parr G, Smith L . Basic matrices for the matrix-assisted laser desorption/ionization mass spectrometry of proteins and oligonucleotides. Anal Chem. 1993; 65(22):3204-11. DOI: 10.1021/ac00070a007. View

Inutan E, Trimpin S . Laserspray ionization (LSI) ion mobility spectrometry (IMS) mass spectrometry. J Am Soc Mass Spectrom. 2010; 21(7):1260-4. DOI: 10.1016/j.jasms.2010.03.039. View

Fenn J, Mann M, Meng C, Wong S, Whitehouse C . Electrospray ionization for mass spectrometry of large biomolecules. Science. 1989; 246(4926):64-71. DOI: 10.1126/science.2675315. View

Lang Z, Guerrera M, Li R, Ladisch S . Ganglioside GD1a enhances VEGF-induced endothelial cell proliferation and migration. Biochem Biophys Res Commun. 2001; 282(4):1031-7. DOI: 10.1006/bbrc.2001.4630. View

Facci L, Skaper S, Favaron M, Leon A . A role for gangliosides in astroglial cell differentiation in vitro. J Cell Biol. 1988; 106(3):821-8. PMC: 2115083. DOI: 10.1083/jcb.106.3.821. View

Ann Q, Adams J . Structure determination of ceramides and neutral glycosphingolipids by collisional activation of [M + Li](+) ions. J Am Soc Mass Spectrom. 2013; 3(3):260-3. DOI: 10.1016/1044-0305(92)87010-V. View

Harvey D . Matrix-assisted laser desorption/ionisation mass spectrometry of oligosaccharides and glycoconjugates. J Chromatogr A. 1996; 720(1-2):429-46. DOI: 10.1016/0021-9673(95)00307-x. View

10.

Nojiri H, Takaku F, Terui Y, Miura Y, Saito M . Ganglioside GM3: an acidic membrane component that increases during macrophage-like cell differentiation can induce monocytic differentiation of human myeloid and monocytoid leukemic cell lines HL-60 and U937. Proc Natl Acad Sci U S A. 1986; 83(3):782-6. PMC: 322949. DOI: 10.1073/pnas.83.3.782. View

11.

Lietz C, Richards A, Ren Y, Trimpin S . Inlet ionization: protein analyses from the solid state without the use of a voltage or a laser producing up to 67 charges on the 66 kDa BSA protein. Rapid Commun Mass Spectrom. 2011; 25(22):3453-6. DOI: 10.1002/rcm.5233. View

12.

Palestini P, Sonnino S, Tettamanti G . Lack of the ganglioside molecular species containing the C20-long-chain bases in human, rat, mouse, rabbit, cat, dog, and chicken brains during prenatal life. J Neurochem. 1991; 56(6):2048-50. DOI: 10.1111/j.1471-4159.1991.tb03465.x. View

13.

Kolter T, Sandhoff K . Sphingolipid metabolism diseases. Biochim Biophys Acta. 2006; 1758(12):2057-79. DOI: 10.1016/j.bbamem.2006.05.027. View

14.

Lopez P, Schnaar R . Gangliosides in cell recognition and membrane protein regulation. Curr Opin Struct Biol. 2009; 19(5):549-57. PMC: 2763983. DOI: 10.1016/j.sbi.2009.06.001. View

15.

Brown D, London E . Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J Biol Chem. 2000; 275(23):17221-4. DOI: 10.1074/jbc.R000005200. View

16.

Jackson S, Wang H, Woods A . In situ structural characterization of glycerophospholipids and sulfatides in brain tissue using MALDI-MS/MS. J Am Soc Mass Spectrom. 2006; 18(1):17-26. DOI: 10.1016/j.jasms.2006.08.015. View

17.

Sugiura Y, Shimma S, Konishi Y, Yamada M, Setou M . Imaging mass spectrometry technology and application on ganglioside study; visualization of age-dependent accumulation of C20-ganglioside molecular species in the mouse hippocampus. PLoS One. 2008; 3(9):e3232. PMC: 2532745. DOI: 10.1371/journal.pone.0003232. View

18.

Zamfir A, Vukelic Z, Bindila L, Peter-Katalinic J, Almeida R, Sterling A . Fully-automated chip-based nanoelectrospray tandem mass spectrometry of gangliosides from human cerebellum. J Am Soc Mass Spectrom. 2004; 15(11):1649-57. DOI: 10.1016/j.jasms.2004.08.002. View

19.

van Remoortere A, van Zeijl R, van den Oever N, Franck J, Longuespee R, Wisztorski M . MALDI imaging and profiling MS of higher mass proteins from tissue. J Am Soc Mass Spectrom. 2010; 21(11):1922-9. DOI: 10.1016/j.jasms.2010.07.011. View

20.

McEwen C, Larsen B, Trimpin S . Laserspray ionization on a commercial atmospheric pressure-MALDI mass spectrometer ion source: selecting singly or multiply charged ions. Anal Chem. 2010; 82(12):4998-5001. DOI: 10.1021/ac1006624. View