Genome-wide High-resolution ACGH Analysis of Gestational Choriocarcinomas

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Jan 19

PMID 22253721

Citations 8

Authors

Henriette Poaty

Philippe Coullin

Jean Felix Peko

Philippe Dessen

Ange Lucien Diatta

Alexander Valent

Eric Leguern

Sophie Prevot

Charles Gombe-Mbalawa

Jean-Jacques Candelier

Jean-Yves Picard

Alain Bernheim

Affiliations

Soon will be listed here.

Abstract

Eleven samples of DNA from choriocarcinomas were studied by high resolution CGH-array 244 K. They were studied after histopathological confirmation of the diagnosis, of the androgenic etiology and after a microsatellite marker analysis confirming the absence of contamination of tumor DNA from maternal DNA. Three cell lines, BeWo, JAR, JEG were also studied by this high resolution pangenomic technique. According to aCGH analysis, the de novo choriocarcinomas exhibited simple chromosomal rearrangements or normal profiles. The cell lines showed various and complex chromosomal aberrations. 23 Minimal Critical Regions were defined that allowed us to list the genes that were potentially implicated. Among them, unusually high numbers of microRNA clusters and imprinted genes were observed.

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References

Keyse S . Dual-specificity MAP kinase phosphatases (MKPs) and cancer. Cancer Metastasis Rev. 2008; 27(2):253-61. DOI: 10.1007/s10555-008-9123-1. View

Deveault C, Qian J, Chebaro W, Ao A, Gilbert L, Mehio A . NLRP7 mutations in women with diploid androgenetic and triploid moles: a proposed mechanism for mole formation. Hum Mol Genet. 2008; 18(5):888-97. DOI: 10.1093/hmg/ddn418. View

Lin S, Coan P, da Rocha S, Seitz H, Cavaille J, Teng P . Differential regulation of imprinting in the murine embryo and placenta by the Dlk1-Dio3 imprinting control region. Development. 2006; 134(2):417-26. DOI: 10.1242/dev.02726. View

Liu L, Channavajhala P, Rao V, Moutsatsos I, Wu L, Zhang Y . Proteomic characterization of the dynamic KSR-2 interactome, a signaling scaffold complex in MAPK pathway. Biochim Biophys Acta. 2009; 1794(10):1485-95. DOI: 10.1016/j.bbapap.2009.06.016. View

Hertz R . Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc Soc Exp Biol Med. 1959; 102:77-81. DOI: 10.3181/00379727-102-25149. View

PATTILLO R, GEY G . The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res. 1968; 28(7):1231-6. View

Iafrate A, Feuk L, Rivera M, Listewnik M, Donahoe P, Qi Y . Detection of large-scale variation in the human genome. Nat Genet. 2004; 36(9):949-51. DOI: 10.1038/ng1416. View

Couillin P, Ravise N, Afoutou J, Chaibi R, Azoulay M, Hors J . [HLA and molar pregnancies (triploidies, hydatidiform moles and choriocarcinoma). Etiological and epidemiological study]. Ann Genet. 1987; 30(4):197-208. View

Ahmed M, Kim K, Haddad B, Berchuck A, Qumsiyeh M . Comparative genomic hybridization studies in hydatidiform moles and choriocarcinoma: amplification of 7q21-q31 and loss of 8p12-p21 in choriocarcinoma. Cancer Genet Cytogenet. 2000; 116(1):10-5. DOI: 10.1016/s0165-4608(99)00103-x. View

10.

Redon R, Ishikawa S, Fitch K, Feuk L, Perry G, Andrews T . Global variation in copy number in the human genome. Nature. 2006; 444(7118):444-54. PMC: 2669898. DOI: 10.1038/nature05329. View

11.

Baudis M . Genomic imbalances in 5918 malignant epithelial tumors: an explorative meta-analysis of chromosomal CGH data. BMC Cancer. 2007; 7:226. PMC: 2225423. DOI: 10.1186/1471-2407-7-226. View

12.

Croucher D, Saunders D, Lobov S, Ranson M . Revisiting the biological roles of PAI2 (SERPINB2) in cancer. Nat Rev Cancer. 2008; 8(7):535-45. DOI: 10.1038/nrc2400. View

13.

Schropfer A, Kammerer U, Kapp M, Dietl J, Feix S, Anacker J . Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines. BMC Cancer. 2010; 10:553. PMC: 2964638. DOI: 10.1186/1471-2407-10-553. View

14.

Beaulieu Bergeron M, Bouron-Dal Soglio D, Maietta A, Fournet J, Blumenkrantz M, Brochu P . Coexistence of a choriocarcinoma and a gonadoblastoma in the gonad of a 46,XY female: a single nucleotide polymorphism array analysis. Pediatr Dev Pathol. 2009; 13(1):66-71. DOI: 10.2350/09-02-0606-CR.1. View

15.

Lee S, Rhee M, Min Y, Kim S . Involvement of peroxiredoxin IV in the 16alpha-hydroxyestrone-induced proliferation of human MCF-7 breast cancer cells. Cell Biol Int. 2008; 32(4):401-5. DOI: 10.1016/j.cellbi.2007.12.009. View

16.

Huang J, Zhang X, Zhang M, Zhu J, Zhang Y, Lin Y . Up-regulation of DLK1 as an imprinted gene could contribute to human hepatocellular carcinoma. Carcinogenesis. 2006; 28(5):1094-103. DOI: 10.1093/carcin/bgl215. View

17.

Lai C, Chan K, Khoo U, Ngan H, Xue W, Chiu P . Analysis of gestational trophoblastic disease by genotyping and chromosome in situ hybridization. Mod Pathol. 2003; 17(1):40-8. DOI: 10.1038/modpathol.3800010. View

18.

Hagan J, ONeill B, Stewart C, Kozlov S, Croce C . At least ten genes define the imprinted Dlk1-Dio3 cluster on mouse chromosome 12qF1. PLoS One. 2009; 4(2):e4352. PMC: 2632752. DOI: 10.1371/journal.pone.0004352. View

19.

Riemenschneider M, Buschges R, Wolter M, Reifenberger J, Bostrom J, Kraus J . Amplification and overexpression of the MDM4 (MDMX) gene from 1q32 in a subset of malignant gliomas without TP53 mutation or MDM2 amplification. Cancer Res. 2000; 59(24):6091-6. View

20.

Li W, Zheng X, Gu J, Ferrell G, Brady M, Esmon N . Extraembryonic expression of EPCR is essential for embryonic viability. Blood. 2005; 106(8):2716-22. PMC: 1895308. DOI: 10.1182/blood-2005-01-0406. View