Hosting Infection: Experimental Models to Assay Candida Virulence

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2012 Jan 12

PMID 22235206

Citations 48

Authors

Donna M MacCallum

Affiliations

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Abstract

Although normally commensals in humans, Candida albicans, Candida tropicalis, Candida parapsilosis, Candida glabrata, and Candida krusei are capable of causing opportunistic infections in individuals with altered physiological and/or immunological responses. These fungal species are linked with a variety of infections, including oral, vaginal, gastrointestinal, and systemic infections, with C. albicans the major cause of infection. To assess the ability of different Candida species and strains to cause infection and disease requires the use of experimental infection models. This paper discusses the mucosal and systemic models of infection available to assay Candida virulence and gives examples of some of the knowledge that has been gained to date from these models.

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References

Andrutis K, Riggle P, Kumamoto C, Tzipori S . Intestinal lesions associated with disseminated candidiasis in an experimental animal model. J Clin Microbiol. 2000; 38(6):2317-23. PMC: 86791. DOI: 10.1128/JCM.38.6.2317-2323.2000. View

Brothers K, Newman Z, Wheeler R . Live imaging of disseminated candidiasis in zebrafish reveals role of phagocyte oxidase in limiting filamentous growth. Eukaryot Cell. 2011; 10(7):932-44. PMC: 3147414. DOI: 10.1128/EC.05005-11. View

Bistoni F, Vecchiarelli A, Cenci E, Sbaraglia G, Perito S, Cassone A . A comparison of experimental pathogenicity of Candida species in cyclophosphamide-immunodepressed mice. Sabouraudia. 1984; 22(5):409-18. DOI: 10.1080/00362178485380661. View

Dunphy G, Oberholzer U, Whiteway M, Zakarian R, Boomer I . Virulence of Candida albicans mutants toward larval Galleria mellonella (Insecta, Lepidoptera, Galleridae). Can J Microbiol. 2003; 49(8):514-24. DOI: 10.1139/w03-064. View

Clemons K, Spearow J, Parmar R, Espiritu M, Stevens D . Genetic susceptibility of mice to Candida albicans vaginitis correlates with host estrogen sensitivity. Infect Immun. 2004; 72(8):4878-80. PMC: 470601. DOI: 10.1128/IAI.72.8.4878-4880.2004. View

Togni G, Sanglard D, Monod M . Acid proteinase secreted by Candida tropicalis: virulence in mice of a proteinase negative mutant. J Med Vet Mycol. 1994; 32(4):257-65. DOI: 10.1080/02681219480000331. View

Chen Z, Kong X . Study of Candida albicans vaginitis model in Kunming mice. J Huazhong Univ Sci Technolog Med Sci. 2007; 27(3):307-10. DOI: 10.1007/s11596-007-0323-7. View

Cantorna M, Balish E . Mucosal and systemic candidiasis in congenitally immunodeficient mice. Infect Immun. 1990; 58(4):1093-100. PMC: 258587. DOI: 10.1128/iai.58.4.1093-1100.1990. View

Grigoriou O, Baka S, Makrakis E, Hassiakos D, Kapparos G, Kouskouni E . Prevalence of clinical vaginal candidiasis in a university hospital and possible risk factors. Eur J Obstet Gynecol Reprod Biol. 2005; 126(1):121-5. DOI: 10.1016/j.ejogrb.2005.09.015. View

10.

Levraud J, Colucci-Guyon E, Redd M, Lutfalla G, Herbomel P . In vivo analysis of zebrafish innate immunity. Methods Mol Biol. 2008; 415:337-63. DOI: 10.1007/978-1-59745-570-1_20. View

11.

Ferrari S, Sanguinetti M, Torelli R, Posteraro B, Sanglard D . Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata. PLoS One. 2011; 6(3):e17589. PMC: 3052359. DOI: 10.1371/journal.pone.0017589. View

12.

Antinori S, Nebuloni M, Magni C, Fasan M, Adorni F, Viola A . Trends in the postmortem diagnosis of opportunistic invasive fungal infections in patients with AIDS: a retrospective study of 1,630 autopsies performed between 1984 and 2002. Am J Clin Pathol. 2009; 132(2):221-7. DOI: 10.1309/AJCPRAAE8LZ7DTNE. View

13.

Hamamoto H, Kurokawa K, Kaito C, Kamura K, Razanajatovo I, Kusuhara H . Quantitative evaluation of the therapeutic effects of antibiotics using silkworms infected with human pathogenic microorganisms. Antimicrob Agents Chemother. 2004; 48(3):774-9. PMC: 353159. DOI: 10.1128/AAC.48.3.774-779.2004. View

14.

Fong I . The rectal carriage of yeast in patients with vaginal candidiasis. Clin Invest Med. 1994; 17(5):426-31. View

15.

Kennedy M, Volz P . Dissemination of yeasts after gastrointestinal inoculation in antibiotic-treated mice. Sabouraudia. 1983; 21(1):27-33. DOI: 10.1080/00362178385380051. View

16.

Budtz-Jorgensen E . Denture stomatitis. IV. An experimental model in monkeys. Acta Odontol Scand. 1971; 29(5):513-26. DOI: 10.3109/00016357109026330. View

17.

Miyazaki T, Inamine T, Yamauchi S, Nagayoshi Y, Saijo T, Izumikawa K . Role of the Slt2 mitogen-activated protein kinase pathway in cell wall integrity and virulence in Candida glabrata. FEMS Yeast Res. 2010; 10(3):343-52. DOI: 10.1111/j.1567-1364.2010.00611.x. View

18.

Calcagno A, Bignell E, Rogers T, Jones M, Muhlschlegel F, Haynes K . Candida glabrata Ste11 is involved in adaptation to hypertonic stress, maintenance of wild-type levels of filamentation and plays a role in virulence. Med Mycol. 2005; 43(4):355-64. DOI: 10.1080/13693780400006088. View

19.

Calcagno A, Bignell E, Rogers T, Canedo M, Muhlschlegel F, Haynes K . Candida glabrata Ste20 is involved in maintaining cell wall integrity and adaptation to hypertonic stress, and is required for wild-type levels of virulence. Yeast. 2004; 21(7):557-68. DOI: 10.1002/yea.1125. View

20.

Kubota M, Kamai Y, Hosokawa T, Fukuoka T, Filler S . New model of oropharyngeal candidiasis in mice. Antimicrob Agents Chemother. 2001; 45(11):3195-7. PMC: 90803. DOI: 10.1128/AAC.45.11.3195-3197.2001. View