Using Transcriptomics and RNA Enrichment to Identify Genes Involved in Virulence of

Overview

Journal Virulence

Specialty Microbiology

Date 2022 Jul 7

PMID 35795910

Authors

Sanne Schrevens

Eric Durandau

Van Du T Tran

Dominique Sanglard

Affiliations

Soon will be listed here.

Abstract

species are the most commonly isolated opportunistic fungal pathogens in humans. causes most of the diagnosed infections, closely followed by . is well studied, and many genes have been shown to be important for infection and colonization of the host. It is however less clear how infects the host. With the help of fungal RNA enrichment, we here investigated for the first time the transcriptomic profile of during urinary tract infection (UTI) in mice. In the UTI model, bladders and kidneys are major target organs and therefore fungal transcriptomes were addressed in these organs. Our results showed that, next to adhesins and proteases, nitrogen metabolism and regulation play a vital role during UTI. Genes involved in nitrogen metabolism were upregulated and among them we show that (urea amidolyase) and (amino acid permease) were important for virulence. Furthermore, we confirmed the importance of the glyoxylate cycle in the host and identified (malate synthase) as an important gene necessary for virulence. In conclusion, our study shows with the support of transcriptomics how adapts to host conditions.

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