CD49f-based Selection of Circulating Tumor Cells (CTCs) Improves Detection Across Breast Cancer Subtypes

Overview

Journal Cancer Lett

Specialty Oncology

Date 2011 Dec 29

PMID 22202642

Citations 25

Authors

Bianca Mostert

Jaco Kraan

Anieta M Sieuwerts

Petra van der Spoel

Joan Bolt-de Vries

Wendy J C Prager-van der Smissen

Marcel Smid

Annemieke M Timmermans

John W M Martens

Jan W Gratama

John A Foekens

Stefan Sleijfer

Affiliations

Soon will be listed here.

Abstract

Circulating tumor cells (CTCs) can be enumerated using CellSearch, but not all breast cancer subtypes, specifically those with epithelial-mesenchymal transition (EMT) characteristics, sufficiently express the enrichment (EpCAM) and selection (CK8/18/19) markers used in this method. While CD146 can detect EpCAM-negative CTCs, we here evaluated the value of various cytokeratins and CD49f to detect CK8/18/19-negative CTCs. The tested cytokeratins provided no substantial benefit, but adding CD49f to CK8/18/19 as a selection marker resulted in improved recovery of normal-like cell lines. Combined staining of CK8/18/19 and CD49f after CD146/EpCAM enrichment is likely to further improve CTC detection in breast cancer.

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