Comprehensive Analysis of the Transcriptional Response of Human Decidual Cells to Lipopolysaccharide Stimulation

Overview

Journal J Reprod Immunol

Specialty Allergy & Immunology

Date 2011 Dec 27

PMID 22196106

Citations 4

Authors

Katherine P Himes

Daniel Handley

Tianjiao Chu

Brian Burke

Kimberly Bunce

Hyagriv N Simhan

David G Peters

Affiliations

Soon will be listed here.

Abstract

Decidual cells are central to innate immunity at the maternal/fetal interface. We sought to characterize the response of decidual cells to stimulation and then removal of lipopolysaccharide (LPS) using a whole genome approach. Decidual cells were isolated from term unlabored cesarean sections. Cells were stimulated with LPS and RNA isolated both pre-stimulation and 2 and 24 h post-stimulation. Media were changed and RNA extracted 48 h later. Gene expression was measured using Agilent 44K whole genome microarrays. Data were visualized and interpreted using Ingenuity Pathway Analysis (IPA) software and selected (n=5) target gene expression was verified with quantitative real-time PCR. Genes related to immune function were up-regulated at 2 and 24 h after LPS exposure and then generally returned to baseline or were at least substantially reduced after LPS removal. Pathway analysis also revealed that genes involved in lipid metabolism (specifically cholesterol and steroid biosynthesis), iron metabolism, and the plasminogen system were coordinately altered following exposure to LPS. Our novel, preliminary findings provide insight into possible mechanisms via which the host inflammatory response could contribute to preterm birth and warrant further investigation in preterm samples.

Citing Articles

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Transcriptome, Spliceosome and Editome Expression Patterns of the Porcine Endometrium in Response to a Single Subclinical Dose of Enteritidis Lipopolysaccharide.

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Decidual stromal cell-derived PGE regulates macrophage responses to microbial threat.

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