» Articles » PMID: 12135912

Nuclear Factor Kappa B Regulation of Proinflammatory Cytokines in Human Gestational Tissues in Vitro

Overview
Journal Biol Reprod
Date 2002 Jul 24
PMID 12135912
Citations 107
Authors
Affiliations
Soon will be listed here.
Abstract

Proinflammatory cytokines are implicated in the initiation and progression of human labor and delivery, particularly in relation to infection-induced preterm labor. In nongestational tissues, the nuclear factor kappa B (NF-kappaB) transcription pathway is a key regulator of proinflammatory cytokine release. In these tissues, sulfasalazine (SASP), through its ability to inhibit NF-kappaB activation, inhibits release of interleukin (IL)-2, IL-12, and tumor necrosis factor (TNF)-alpha. Therefore, the aim of this study was to investigate whether or not NF-kappaB activation regulates the formation of proinflammatory cytokines in human gestational tissues. Human placenta, amnion, and choriodecidua (n = 9 separate placentas) were incubated with 10 microg/ml of lipopolysaccharide (LPS) in the absence (control) or presence of SASP (0.1, 1, 5, or 10 mM). After 6 h of incubation, the tissues were collected, and NF-kappaB DNA binding activity in nuclear extracts was assessed by electromobility shift binding assay. The incubation medium was collected and the release of IL-6, IL-8, and TNF-alpha was quantified by ELISA. Treatment of placenta, amnion, and choriodecidua with SASP at concentrations 5 mM or greater significantly inhibited the release of IL-6, IL-8, and TNF-alpha, and NF-kappaB activation (ANOVA, P < 0.05). The data presented in this study demonstrate that the NF-kappaB transcription pathway is a key regulator of LPS-stimulated IL-6, IL-8, and TNF-alpha release from human gestational tissues. The control of NF-kappaB activation may therefore provide an alternative therapeutic strategy for reducing the release of proinflammatory mediators in infection associated preterm labor.

Citing Articles

Cirsimaritin Alleviates Dextran Sodium Sulfate-Induced Acute Colitis in Experimental Animals: A Therapeutic Approach for Inflammatory Bowel Disease.

Alqudah A, Qnais E, Gammoh O, Bseiso Y, Wedyan M, Alqudah M Prev Nutr Food Sci. 2024; 29(1):31-39.

PMID: 38576881 PMC: 10987388. DOI: 10.3746/pnf.2024.29.1.31.


Maternal Influenza and Offspring Neurodevelopment.

Egorova M, Egorov V, Zabrodskaya Y Curr Issues Mol Biol. 2024; 46(1):355-366.

PMID: 38248325 PMC: 10814929. DOI: 10.3390/cimb46010023.


Trichinella spiralis cathepsin L induces macrophage M1 polarization via the NF-κB pathway and enhances the ADCC killing of newborn larvae.

Liu R, Meng X, Li C, Xu Q, Lin X, Dong B Parasit Vectors. 2023; 16(1):433.

PMID: 37993938 PMC: 10666456. DOI: 10.1186/s13071-023-06051-1.


Fermentation of Extract with Halophilic CP6 Results in Enhanced Anti-Inflammatory Activities.

Park M, Yeom Y, Ganbat D, Kim M, Kim S, Lee Y Nutrients. 2023; 15(2).

PMID: 36678181 PMC: 9864326. DOI: 10.3390/nu15020309.


An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure.

Nakamura H, Kimura T Biomolecules. 2023; 13(1).

PMID: 36671464 PMC: 9856033. DOI: 10.3390/biom13010079.