Determination of Human Anticytokine Autoantibody Profiles Using a Particle-based Approach

Overview

Journal J Clin Immunol

Publisher Springer

Specialty Allergy & Immunology

Date 2011 Dec 16

PMID 22170314

Citations 35

Authors

Li Ding

Allen Mo

Kamonwan Jutivorakool

Minjal Pancholi

Steven M Holland

Sarah K Browne

Affiliations

Soon will be listed here.

Abstract

Background: Anticytokine autoantibodies cause numerous human diseases, ranging from pure red cell aplasia to acquired immunodeficiencies. Rapid, simple, and affordable detection and monitoring of these antibodies is essential. We sought to develop a standardizable assay that is rapid, sensitive, and specific and able to simultaneously detect multiple anticytokine autoantibodies in small volumes (<10 μl).

Methods: We conjugated purified human cytokines to commercially available fluorescently labeled microspheres and tested them against sera from well-characterized subjects with at least one high-titer, disease-associated anticytokine autoantibody.

Results: Cytokine-conjugated microspheres efficiently and rapidly determined plasma concentration and IgG subclass of anticytokine autoantibodies in single or multiplex formats.

Conclusion: This particle-based multiplex assay can reproducibly characterize anticytokine autoantibodies. This efficient and inexpensive approach to diagnosing and monitoring anticytokine autoantibodies has clinical applications.

Citing Articles

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Autoantibodies to Interferons in Infectious Diseases.

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