Stacked Graphene-Al2O3 Nanopore Sensors for Sensitive Detection of DNA and DNA-protein Complexes

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2011 Dec 15

PMID 22165962

Citations 51

Authors

Bala Murali Venkatesan

David Estrada

Shouvik Banerjee

Xiaozhong Jin

Vincent E Dorgan

Myung-Ho Bae

Narayana R Aluru

Eric Pop

Rashid Bashir

Affiliations

Soon will be listed here.

Abstract

We report the development of a multilayered graphene-Al(2)O(3) nanopore platform for the sensitive detection of DNA and DNA-protein complexes. Graphene-Al(2)O(3) nanolaminate membranes are formed by sequentially depositing layers of graphene and Al(2)O(3), with nanopores being formed in these membranes using an electron-beam sculpting process. The resulting nanopores are highly robust, exhibit low electrical noise (significantly lower than nanopores in pure graphene), are highly sensitive to electrolyte pH at low KCl concentrations (attributed to the high buffer capacity of Al(2)O(3)), and permit the electrical biasing of the embedded graphene electrode, thereby allowing for three terminal nanopore measurements. In proof-of-principle biomolecule sensing experiments, the folded and unfolded transport of single DNA molecules and RecA-coated DNA complexes could be discerned with high temporal resolution. The process described here also enables nanopore integration with new graphene-based structures, including nanoribbons and nanogaps, for single-molecule DNA sequencing and medical diagnostic applications.

Citing Articles

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