Nanopore Sensors for Nucleic Acid Analysis

Overview

Journal Nat Nanotechnol

Specialty Biotechnology

Date 2011 Sep 20

PMID 21926981

Citations 303

Authors

Bala Murali Venkatesan

Rashid Bashir

Affiliations

Soon will be listed here.

Abstract

Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resolution and without the need for labels or amplification. Recent advances suggest that nanopore-based sensors could be competitive with other third-generation DNA sequencing technologies, and may be able to rapidly and reliably sequence the human genome for under $1,000. In this article we review the use of nanopore technology in DNA sequencing, genetics and medical diagnostics.

Citing Articles

Detecting and Controlling DNA Translocation through a Nanopore using a van der Waals Heterojunction Diode.

Chen S, Huang S, Son J, Han E, Watanabe K, Taniguchi T Res Sq. 2024; .

PMID: 39606466 PMC: 11601842. DOI: 10.21203/rs.3.rs-5193820/v2.

Langevin Dynamics Study on the Driven Translocation of Polymer Chains with a Hairpin Structure.

Wu F, Yang X, Wang C, Zhao B, Luo M Molecules. 2024; 29(17).

PMID: 39274890 PMC: 11397710. DOI: 10.3390/molecules29174042.

Multi-physics simulations and experimental comparisons for the optical and electrical forces acting on a silica nanoparticle trapped by a double-nanohole plasmonic nanopore sensor.

Asadzadeh H, Renkes S, Kim M, Alexandrakis G Sens Biosensing Res. 2024; 41.

PMID: 39239382 PMC: 11376433. DOI: 10.1016/j.sbsr.2023.100581.

Immunological assay using a solid-state pore with a low limit of detection.

Takei H, Nakada T, Leong L, Ito A, Hanada K, Maeda H Sci Rep. 2024; 14(1):16686.

PMID: 39030274 PMC: 11271571. DOI: 10.1038/s41598-024-67112-8.

Micro- and Nanofluidic pH Sensors Based on Electrodiffusioosmosis.

Takagi T, Kishimoto T, Doi K Micromachines (Basel). 2024; 15(6).

PMID: 38930669 PMC: 11205811. DOI: 10.3390/mi15060698.

References

Hoogerheide D, Garaj S, Golovchenko J . Probing surface charge fluctuations with solid-state nanopores. Phys Rev Lett. 2009; 102(25):256804. PMC: 2865846. DOI: 10.1103/PhysRevLett.102.256804. View

. A haplotype map of the human genome. Nature. 2005; 437(7063):1299-320. PMC: 1880871. DOI: 10.1038/nature04226. View

Olasagasti F, Lieberman K, Benner S, Cherf G, Dahl J, Deamer D . Replication of individual DNA molecules under electronic control using a protein nanopore. Nat Nanotechnol. 2010; 5(11):798-806. PMC: 3711841. DOI: 10.1038/nnano.2010.177. View

Lagerqvist J, Zwolak M, Di Ventra M . Fast DNA sequencing via transverse electronic transport. Nano Lett. 2006; 6(4):779-82. PMC: 2556950. DOI: 10.1021/nl0601076. View

Yusko E, Johnson J, Majd S, Prangkio P, Rollings R, Li J . Controlling protein translocation through nanopores with bio-inspired fluid walls. Nat Nanotechnol. 2011; 6(4):253-60. PMC: 3071889. DOI: 10.1038/nnano.2011.12. View

Geim A . Graphene: status and prospects. Science. 2009; 324(5934):1530-4. DOI: 10.1126/science.1158877. View

Merchant C, Healy K, Wanunu M, Ray V, Peterman N, Bartel J . DNA translocation through graphene nanopores. Nano Lett. 2010; 10(8):2915-21. DOI: 10.1021/nl101046t. View

Langford K, Penkov B, Derrington I, Gundlach J . Unsupported planar lipid membranes formed from mycolic acids of Mycobacterium tuberculosis. J Lipid Res. 2010; 52(2):272-7. PMC: 3023547. DOI: 10.1194/jlr.M012013. View

Storm A, Storm C, Chen J, Zandbergen H, Joanny J, Dekker C . Fast DNA translocation through a solid-state nanopore. Nano Lett. 2005; 5(7):1193-7. DOI: 10.1021/nl048030d. View

10.

Cockroft S, Chu J, Amorin M, Ghadiri M . A single-molecule nanopore device detects DNA polymerase activity with single-nucleotide resolution. J Am Chem Soc. 2008; 130(3):818-20. PMC: 2453067. DOI: 10.1021/ja077082c. View

11.

Ivanov A, Instuli E, McGilvery C, Baldwin G, McComb D, Albrecht T . DNA tunneling detector embedded in a nanopore. Nano Lett. 2010; 11(1):279-85. PMC: 3020087. DOI: 10.1021/nl103873a. View

12.

Li J, Gershow M, Stein D, Brandin E, Golovchenko J . DNA molecules and configurations in a solid-state nanopore microscope. Nat Mater. 2003; 2(9):611-5. DOI: 10.1038/nmat965. View

13.

Heng J, Aksimentiev A, Ho C, Dimitrov V, Sorsch T, Miner J . Beyond the Gene Chip. Bell Labs Tech J. 2008; 10(3):5-22. PMC: 2546600. DOI: 10.1002/bltj.20102. View

14.

Iqbal S, Akin D, Bashir R . Solid-state nanopore channels with DNA selectivity. Nat Nanotechnol. 2008; 2(4):243-8. DOI: 10.1038/nnano.2007.78. View

15.

Chung M, Boxer S . Stability of DNA-tethered lipid membranes with mobile tethers. Langmuir. 2011; 27(9):5492-7. PMC: 3085013. DOI: 10.1021/la200234h. View

16.

Wanunu M, Sutin J, McNally B, Chow A, Meller A . DNA translocation governed by interactions with solid-state nanopores. Biophys J. 2008; 95(10):4716-25. PMC: 2576395. DOI: 10.1529/biophysj.108.140475. View

17.

Rincon-Restrepo M, Mikhailova E, Bayley H, Maglia G . Controlled translocation of individual DNA molecules through protein nanopores with engineered molecular brakes. Nano Lett. 2011; 11(2):746-50. PMC: 3391008. DOI: 10.1021/nl1038874. View

18.

Hall A, Scott A, Rotem D, Mehta K, Bayley H, Dekker C . Hybrid pore formation by directed insertion of α-haemolysin into solid-state nanopores. Nat Nanotechnol. 2010; 5(12):874-7. PMC: 3137937. DOI: 10.1038/nnano.2010.237. View

19.

Astier Y, Braha O, Bayley H . Toward single molecule DNA sequencing: direct identification of ribonucleoside and deoxyribonucleoside 5'-monophosphates by using an engineered protein nanopore equipped with a molecular adapter. J Am Chem Soc. 2006; 128(5):1705-10. DOI: 10.1021/ja057123+. View

20.

White R, Zhang B, Daniel S, Tang J, Ervin E, Cremer P . Ionic conductivity of the aqueous layer separating a lipid bilayer membrane and a glass support. Langmuir. 2006; 22(25):10777-83. DOI: 10.1021/la061457a. View