Cellular Delivery of PEGylated PLGA Nanoparticles

Overview

Journal J Pharm Pharmacol

Publisher Oxford University Press

Specialties Pharmacology
Pharmacy

Date 2011 Dec 14

PMID 22150673

Citations 24

Authors

Sarala Pamujula

Sidhartha Hazari

Gevoni Bolden

Richard A Graves

Dakshinamurthy Devanga Chinta

Srikanta Dash

Vimal Kishore

Tarun K Mandal

Affiliations

Soon will be listed here.

Abstract

Objectives: The objective of this study was to investigate the efficiency of uptake of PEGylated polylactide-co-gycolide (PLGA) nanoparticles by breast cancer cells.

Methods: Nanoparticles of PLGA containing various amounts of polyethylene glycol (PEG, 5%-15%) were prepared using a double emulsion solvent evaporation method. The nanoparticles were loaded with coumarin-6 (C6) as a fluorescence marker. The particles were characterized for surface morphology, particle size, zeta potential, and for cellular uptake by 4T1 murine breast cancer cells.

Key Findings: Irrespective of the amount of PEG, all formulations yielded smooth spherical particles. However, a comparison of the particle size of various formulations showed bimodal distribution of particles. Each formulation was later passed through a 1.2 µm filter to obtain target size particles (114-335 nm) with zeta potentials ranging from -2.8 mV to -26.2 mV. While PLGA-PEG di-block (15% PEG) formulation showed significantly higher 4T1 cellular uptake than all other formulations, there was no statistical difference in cellular uptake among PLGA, PLGA-PEG-PLGA tri-block (10% PEG), PLGA-PEG di-block (5% PEG) and PLGA-PEG di-block (10% PEG) nanoparticles.

Conclusion: These preliminary findings indicated that the nanoparticle formulation prepared with 15% PEGylated PLGA showed maximum cellular uptake due to it having the smallest particle size and lowest zeta potential.

Citing Articles

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Kosksi T, Bustos-Salgado P, Rejeb M, Selmi A, Debbabi N, Espinoza L Int J Mol Sci. 2025; 26(5).

PMID: 40076748 PMC: 11901007. DOI: 10.3390/ijms26052124.

Application of PLGA-PEG-PLGA Nanoparticles to Percutaneous Immunotherapy for Food Allergy.

Sakurai R, Iwata H, Gotoh M, Ogino H, Takeuchi I, Makino K Molecules. 2024; 29(17).

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Application of PLGA in Tumor Immunotherapy.

Wu J, Wang X, Wang Y, Xun Z, Li S Polymers (Basel). 2024; 16(9).

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Development of High-Loading Trastuzumab PLGA Nanoparticles: A Powerful Tool Against HER2 Positive Breast Cancer Cells.

Caputo T, Barisciano G, Mule C, Cusano A, Aliberti A, Muccillo L Int J Nanomedicine. 2023; 18:6999-7020.

PMID: 38034948 PMC: 10683664. DOI: 10.2147/IJN.S429898.

Therapeutic applications of nanobiotechnology.

Dutt Y, Pandey R, Dutt M, Gupta A, Vibhuti A, Vidic J J Nanobiotechnology. 2023; 21(1):148.

PMID: 37149615 PMC: 10163736. DOI: 10.1186/s12951-023-01909-z.

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