CD4 is Retained in the Endoplasmic Reticulum by the Human Immunodeficiency Virus Type 1 Glycoprotein Precursor

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Nov 1

PMID 2214026

Citations 103

Authors

B Crise

L Buonocore

J K Rose

Affiliations

Soon will be listed here.

Abstract

We analyzed coexpression of the human immunodeficiency virus type 1 glycoprotein precursor, gp160, and its cellular receptor CD4 in HeLa cells to determine whether the two molecules can interact prior to transport to the cell surface. Results of studies employing coprecipitation, analysis of oligosaccharide processing, and immunocytochemistry showed that newly synthesized CD4 and gp160 form a complex prior to transport from the endoplasmic reticulum (ER). CD4 expressed by itself was transported efficiently from the ER to the cell surface, but the complex of CD4 and gp160 was retained in the ER. This retention of CD4 within the ER is probably a consequence of the very inefficient transport of gp160 itself (R. L. Willey, J. S. Bonifacino, B. J. Potts, M. A. Martin, and R. D. Klausner, Proc. Natl. Acad. Sci. USA 85:9580-9584, 1988). Retention of CD4 in the ER by gp160 may partially explain the down regulation of CD4 in human immunodeficiency virus type 1-infected T cells. Inhibition of CD4 transport appears to be a consequence of the interaction of two membrane-bound molecules, because a complex of CD4 and gp120 (the soluble extracellular domain of gp160) was transported rapidly and efficiently from the ER.

Citing Articles

Double-Negative T-Cells during Acute Human Immunodeficiency Virus and Simian Immunodeficiency Virus Infections and Following Early Antiretroviral Therapy Initiation.

Yero A, Shi T, Clain J, Zghidi-Abouzid O, Racine G, Costiniuk C Viruses. 2024; 16(10).

PMID: 39459942 PMC: 11512404. DOI: 10.3390/v16101609.

Role of Viral Protein U (Vpu) in HIV-1 Infection and Pathogenesis.

Khan N, Geiger J Viruses. 2021; 13(8).

PMID: 34452331 PMC: 8402909. DOI: 10.3390/v13081466.

HIV Infection and Persistence in Pulmonary Mucosal Double Negative T Cells .

Meziane O, Salahuddin S, Pham T, Farnos O, Pagliuzza A, Olivenstein R J Virol. 2020; 94(24).

PMID: 32967958 PMC: 7925170. DOI: 10.1128/JVI.01788-20.

Susceptibility to Neutralization by Broadly Neutralizing Antibodies Generally Correlates with Infected Cell Binding for a Panel of Clade B HIV Reactivated from Latent Reservoirs.

Ren Y, Korom M, Truong R, Chan D, Huang S, Kovacs C J Virol. 2018; 92(23).

PMID: 30209173 PMC: 6232479. DOI: 10.1128/JVI.00895-18.

HIV Genome-Wide Protein Associations: a Review of 30 Years of Research.

Li G, De Clercq E Microbiol Mol Biol Rev. 2016; 80(3):679-731.

PMID: 27357278 PMC: 4981665. DOI: 10.1128/MMBR.00065-15.

References

Buonocore L, Rose J . Prevention of HIV-1 glycoprotein transport by soluble CD4 retained in the endoplasmic reticulum. Nature. 1990; 345(6276):625-8. DOI: 10.1038/345625a0. View

Pelchen-Matthews A, Armes J, Marsh M . Internalization and recycling of CD4 transfected into HeLa and NIH3T3 cells. EMBO J. 1989; 8(12):3641-9. PMC: 402046. DOI: 10.1002/j.1460-2075.1989.tb08538.x. View

Dunphy W, Rothman J . Compartmental organization of the Golgi stack. Cell. 1985; 42(1):13-21. DOI: 10.1016/s0092-8674(85)80097-0. View

Maddon P, Littman D, Godfrey M, Maddon D, Chess L, Axel R . The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family. Cell. 1985; 42(1):93-104. DOI: 10.1016/s0092-8674(85)80105-7. View

KORNFELD R, Kornfeld S . Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985; 54:631-64. DOI: 10.1146/annurev.bi.54.070185.003215. View

McDougal J, Kennedy M, Sligh J, Cort S, Mawle A, Nicholson J . Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule. Science. 1986; 231(4736):382-5. DOI: 10.1126/science.3001934. View

Lifson J, Reyes G, McGrath M, Stein B, Engleman E . AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen. Science. 1986; 232(4754):1123-7. DOI: 10.1126/science.3010463. View

Puddington L, Machamer C, Rose J . Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane. J Cell Biol. 1986; 102(6):2147-57. PMC: 2114239. DOI: 10.1083/jcb.102.6.2147. View

Lasky L, Groopman J, Fennie C, Benz P, Capon D, Dowbenko D . Neutralization of the AIDS retrovirus by antibodies to a recombinant envelope glycoprotein. Science. 1986; 233(4760):209-12. DOI: 10.1126/science.3014647. View

10.

Sodroski J, Goh W, Rosen C, Campbell K, Haseltine W . Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicity. Nature. 1986; 322(6078):470-4. DOI: 10.1038/322470a0. View

11.

Maddon P, Dalgleish A, McDougal J, Clapham P, Weiss R, Axel R . The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell. 1986; 47(3):333-48. DOI: 10.1016/0092-8674(86)90590-8. View

12.

Lifson J, Feinberg M, Reyes G, Rabin L, Banapour B, Chakrabarti S . Induction of CD4-dependent cell fusion by the HTLV-III/LAV envelope glycoprotein. Nature. 1986; 323(6090):725-8. DOI: 10.1038/323725a0. View

13.

Fuerst T, Niles E, Studier F, Moss B . Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A. 1986; 83(21):8122-6. PMC: 386879. DOI: 10.1073/pnas.83.21.8122. View

14.

Hoxie J, Alpers J, Rackowski J, Huebner K, Haggarty B, Cedarbaum A . Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV. Science. 1986; 234(4780):1123-7. DOI: 10.1126/science.3095925. View

15.

Munro S, Pelham H . A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987; 48(5):899-907. DOI: 10.1016/0092-8674(87)90086-9. View

16.

Lyerly H, Matthews T, Langlois A, Bolognesi D, Weinhold K . Human T-cell lymphotropic virus IIIB glycoprotein (gp120) bound to CD4 determinants on normal lymphocytes and expressed by infected cells serves as target for immune attack. Proc Natl Acad Sci U S A. 1987; 84(13):4601-5. PMC: 305138. DOI: 10.1073/pnas.84.13.4601. View

17.

Doyle C, Strominger J . Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature. 1987; 330(6145):256-9. DOI: 10.1038/330256a0. View

18.

JAMIESON J, Palade G . Intracellular transport of secretory proteins in the pancreatic exocrine cell. 3. Dissociation of intracellular transport from protein synthesis. J Cell Biol. 1968; 39(3):580-8. PMC: 2107546. DOI: 10.1083/jcb.39.3.580. View

19.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

20.

Tarentino A, Maley F . Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus. J Biol Chem. 1974; 249(3):811-7. View