Neutralization of the AIDS Retrovirus by Antibodies to a Recombinant Envelope Glycoprotein

Overview

Journal Science

Specialty Science

Date 1986 Jul 11

PMID 3014647

Citations 138

Authors

L A Lasky

J E Groopman

C W Fennie

P M Benz

D J Capon

D J Dowbenko

G R NAKAMURA

W M Nunes

M E Renz

P W BERMAN

Affiliations

Soon will be listed here.

Abstract

Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.

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