Directed Molecular Evolution to Design Advanced Red Fluorescent Proteins

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2011 Dec 1

PMID 22127219

Citations 39

Authors

Fedor V Subach

Kiryl D Piatkevich

Vladislav V Verkhusha

Affiliations

Soon will be listed here.

Abstract

Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins. We discuss possibilities for knowledge-based rational design based on the photochemistry of fluorescent proteins and the position of the chromophore in protein structure. We consider advances in library design by mutagenesis, protein expression systems and instrumentation for high-throughput screening that should yield improved fluorescent proteins for advanced imaging applications.

Citing Articles

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Dual-expression system for blue fluorescent protein optimization.

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Rapid directed molecular evolution of fluorescent proteins in mammalian cells.

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LSSmScarlet, dCyRFP2s, dCyOFP2s and CRISPRed2s, Genetically Encoded Red Fluorescent Proteins with a Large Stokes Shift.

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